Study On The Relationship Between Fat And Obesity-Related Genes,Leptin And Fat Deposition In Mice And Its Regulation Mechanism

Fat and obesity-related genes(FTO)can affect the formation of body fat and energy balance.Leptin is produced by adipose tissue and plays an important role in fat formation and lipid accumulation.Both FTO and Leptin genes are closely related to adipose tissue and play a role in energy metabolism in the body.Therefore,whether there is a link between the two in fat metabolism and interactions needs further study.The association between the FTO gene and the Leptin gene in the JAk-STAT3 signaling pathway and the leptin receptor(Lep R)has been reported.However,the direct link between FTO and Leptin genes in adipocytes has not been reported.This study mainly studied the expression of FTO and Leptin genes in different parts of mice’s adipose tissue under different feeding conditions.At the same time,combined with adipocyte culture experiments to explore the regularity and fat metabolism between FTO and Leptin genes.Contact.It lays a foundation for the connection of FTO and Leptin genes in tail fat of Altaya Taiwei.1 Effects of Different Feeding Conditions on FTO and Leptin Genes in Adipose Tissues of Mice40 Kunming mice(SPF grade,male)were randomly divided into high-fat group(HF,n=20),control group(CF,n=10),limited-feeding group(limited food intake,LF,n=10).were fed high-fat materials,ordinary feed and limited food intake(60% of the control group).After 4 weeks of continuous feeding,after the feeding was completed,blood,epididymal fat,perirenal fat,and subcutaneous fat were weighed and used to study blood biochemical parameters,fat morphological characteristics and gene expression.The cell area of adipose tissue was determined by HE staining.The m RNA and protein expression of FTO and Leptin in adipose tissue were measured by real-time fluorescent quantitative PCR and Western blotting.The results showed: 1.At the fourth week,the mice had a body weight HF of 51.29 ± 3.24 g,a CF of40.07 ± 2.56 g and an LF of 35.42 ± 1.96 g.Compared with the CF group,the HF group had a significant increase(P < 0.01).LF group was significantly lower(P < 0.05).The mass of epididymis,subcutaneous and perirenal fat in HF group was significantly higher than that in CF group(P < 0.01),and the epididymal fat mass in LF group was significantly lower than CF group(P < 0.01).2.Serum TG in HF group was significantly higher than CF group(P < 0.05),T-CHO,LDL-C were significantly higher than CF group(P < 0.01),HDL-C was significantly lower than CF group(P < 0.01).The epididymal fat and the area of peripheral lipid cells in the HF group were significantly greater than those in the CF group(P < 0.05).3.Under high fat intake,the expression of FTO m RNA and protein in the HF group was significantly lower than that in the CF group(P < 0.01)in the epididymis,subcutaneous and perirenal fat,and the Leptin m RNA and protein expression levels were significantly higher than those in the CF group.In the group(P < 0.01),FTO m RNA,protein,and Leptin m RNA expression in the fat of various parts of the LF group were significantly lower than those of the CF group(P < 0.01),as the body weight and body fat decreased.The expression level of Leptin protein was significantly higher than that of CF group(P < 0.01).The results showed that when fed with high-fat diet,the expression of FTO gene was negatively correlated with body weight and fat mass,and the expression of Leptin gene was positively correlated.Feed restriction decreased the expression of FTO and Leptin,FTO and Leptin gene expression was positively correlated with body weight and fat mass.Related.2 Effect of Leptin Antagonist on FTO Expression in 3T3-L1 AdipocytesAt present,studies on the association between FTO and Leptin genes have focused on JAK-STAT3 signaling pathways and Lep R receptors in liver cells and myocardium.There has been no report on the link between FTO and leptin genes in adipocytes.Therefore,in the adipocyte assay,we added leptin antagonists for 48 hours to inhibit Leptin binding to Lep R and to investigate the association between FTO and Leptin genes and lipid metabolism in adipocytes.Oil red O-isopropanol extraction method was used to determine the fat content in cells.Real-time fluorescence quantitative PCR and Western blotting were used to determine the expression of FTO and Leptin in adipocytes.The results showed t: 1.After adding antagonists for 48 h,the cell density in the leptin antagonist group was lower than that in the control group and the fat content was significantly lower than that in the control group(P < 0.01).2.There was no significant difference in Leptin m RNA and protein expression between the Leptin antagonist group and the control group(P > 0.05).However,the expression level of FTO m RNA and protein in the leptin antagonist group was significantly lower than that in the control group(P < 0.05).The results showed that Leptin antagonists blocked the binding of leptin to its receptor,resulting in a decrease in fat content and a down-regulation of FTO expression.The results of mouse and cell experiments show that the expression of Leptin and FTO are related to body fat deposition,and Leptin may have the function of regulating FTO expression during body fat deposition.