Determination And Analysis Of Flavonoids In Flower Buds Of Hemerocallis Spp.

Hemerocallis is a member of the Liliaceae family.It is a perennial perennial herbaceous plant.There are 14 species of this genus,and about 10 species are native to China.They are widely cultivated throughout China.A few species are currently distributed in Russia,Japan,Mongolia,and North Korea and other countries.The flower organ of Hemerocallis is of ornamental,medicinal and edible value.Flavonoids are secondary metabolites of plants with a wide range of biological activities.They have strong pharmacological effects in the aspects of antioxidant,scavenging free radicals,preventing arteriosclerosis and cancer,regulating endocrine,antibacterial and analgesic,etc.Contains secondary metabolites such as flavonoids,sugars,alkaloids,saponins,and phenols,among which the flavonoid content is extremely rich,about 0.5 ~ 100 mg / g.In this study,70 kinds of flower buds of Hemerocallis were used as materials.First,the flavonoids were initially determined and analyzed by high-performance liquid chromatography.The ultrasonic-assisted extraction technology was used to analyze the ultrasonic extraction time,liquid-material ratio,sample quality,and solvent volume fraction.The influence of several factors on the extraction rate of total flavonoids,and the response surface test was designed to optimize the extraction conditions,and a micro-extraction system of flavonoids in the flower buds of Hemerocallis was established.The screening of germplasm resources with high flavonoid content provides a basis for the extraction and determination of flavonoids in the flower buds of Hemerocallis,and provides a germplasm basis for the study of screening for high flavonoids in Hemerocallis species.The research results are as follows:(1)The flavonoids were extracted by the method established by the research group,and six flavonoids were separated by high-performance liquid chromatography,namely rutin,quercetin 3-O-glucoside,myricetin,quercetin,Apigenin and geraniol,the retention times are 23.417 min,24.510 min,29.370 min,34.327 min,38.013 min,38.903 min.Among the six types of flavonoids,the content of rutin is the highest in the range of 105.28 ~ 2674.45 μg / g.Four types of germplasm resources with high flavonoid content were selected: H0086,H0087,H0059,H0060.(2)The best conditions for single factor test are: ultrasonic extraction time 15 min,liquid-to-material ratio 10: 1(m L / g),sample mass 0.2 g,ethanol volume fraction 70%.The optimal extraction conditions of flavonoids from the response surface test are: ultrasonic extraction time 17 min,liquid-to-material ratio 12:1(m L / g),sample mass 0.25 g,ethanol volume fraction 72%,measured by this micro extraction system The average content of total flavonoids in H.’Datong Huanghua’ buds was 4.46 mg / g,which was close to the predicted value.The parents of the current major hybrid group H0006,H0039,H0065 were selected to compare the methods.The results showed that the contents of the six types of flavonoids extracted by the micro-extraction system were significantly(P <0.05)high Based on the method of the research group,a micro-extraction system of flavonoids from flower buds of Hemerocallis was established,which was used for the mass detection of flavonoids from Hemerocallis.(3)The flavonoids in the flower buds of 70 species of Hemerocallis extracted by the micro-extraction system showed that the total flavonoid content of the flower buds of 70 species of Hemerocallis was 2.27 mg/ g ~ 20.79 mg / g,and the material with the highest total flavonoid content was H0092 is 20.79 mg / g,the lowest is H0122,which is 2.27 mg / g.Rutin,quercetin 3-O-glucoside,myricetin,quercetin,apigenin,and geranin have the most abundant rutin content,ranging from 198.47 to 3349.02 μg / g.Four types of germplasm resources with high flavonoid content were screened out: H0086,H0087,H0059,H0190.(5)The high-flavonoid germplasms screened by the method of the research group and the microextraction system are not much different.The common germplasms include H0059,H0086 and H0087.The total flavonoid content is also in the top 50%,so it can be used for the further development of flavonoids and the research on the cultivation of high flavonoid germplasm resources.