The Research On Duck Oil Improves Oxidative Stress Injury Induced By D-Galactose In Mice And Preliminary Exploration Of Its Mechanism

Duck oil contains 30.63%oleic acid,linoleic acid 17.2%,?-linolenic acid 0.37%,and arachidonic acid 0.9%.The unsaturated fatty acids of duck oil is high,which has better utilization value,but the research on duck oil is less at present.Therefore,the ORAC value,FRAP value,DPPH·scavenging rate,the ability to inhibit?-carotene fading,the effect on BSA protein carbonyl and DNA protection potential of duck oil were measured in vitro to evaluate whether duck oil has antioxidant capacity.In animal experiments,mice were injected with D-gal(1000 mg/kg·BW)for 21 days to establish an oxygen stress model,and then gavage with 45 days,low-dose duck oil,medium-dose duck oil(1250 mg/kg·BW),high-dose duck oil(2500 mg/kg·BW),pure duck oil(2500mg/kg·BW),GSH(200 mg/kg·BW),the preparation of duck oil solvent for 45 days.After gavage,the levels of lipid oxidation products,protein oxidation products,DNA oxidation products,antioxidant enzymes,DNA repair enzymes and T-AOC in mice plasma were measured to evaluate whether duck oil can improve oxidative stress injury in mice.Finally,the relative expression of keap1 m RNA,Nrf2 m RNA,SOD m RNA,GPx m RNA,MT m RNA,Trx m RNA,OGG1 m RNA and MTH1 m RNA in mice liver were measured to preliminarily explore the mechanism of duck oil improving oxidative stress injury.The experimental results are as follows:(1)In vitro,the half maximum inhibitory concentration(IC₅₀)for DPPH radical scavenging was(15.03±0.53)mg/m L.when the concentration of duck lipids was 35mg/m L,FRAP value was(241.87±3.05)?mol/L,ORAC value was(2.59±0.15)?mol TE/g,the inhibition rate of?-carotene fading was(67.29±3.37)%.the DNA protection rate of 1 mg/m L duck oil was(43.27±0.01)%.When the concentration of duck oil was 6 m L/g protein,the carbonyl content of BSA increased(50.00±3.21)%,which promoted the oxidation of BSA.(2)Compared with the model group,the content of 8-iso-PGF2?in the plasma of male and female mice fed with high dose duck oil(2500 mg/kg·BW)decreased by20.88%and 16.33%(p<0.05),respectively;the protein oxidation products PCO decreased by 9.91%and 12.06%(p<0.05),3-NT decreased by 11.61%and 13.84%(p<0.05),AOPP decreased by 13.57%and 10.93%(p<0.05);the DNA oxidation products 8-OHd G decreased by 21.48%and 22.87%(p<0.05),5-OHd C decreased by15.10%and 10.04%(p<0.05);the activity of antioxidant enzymes T-SOD increased by30.93%and 45.85%(p<0.05),respectively,GPx activity increased by 24.14%and11.29%(p<0.05);the content of antioxidants MT increased by 17.70%and 9.86%(p<0.05),Trx increased by 24.13%and 21.39%(p<0.05);the DNA repair enzyme activity OGG1 increased by 15.10%and 12.33%(p<0.05),MTH1 increased by 18.27%and9.24%(p<0.05);the T-AOC increased by 23.08%and 27.57%(p<0.05),duck oil improved oxidative stress in mice.There was no significant difference in T-AOC,GPx enzyme activity and the content of Trx,PCO,3-NT,AOPP,8-OHd G,5-OHd C between male and female.The T-SOD activity of mice in low-dose group,middle-dose group and pure duck oil group was significantly different between male and female(p<0.05).The MT content,8-iso-PGF2?content,and MTH1 activity of mice in the high-dose duck oil group were significantly different between male and female(p<0.05).The OGG1activity of the model group mice was significantly different between male and female(p<0.05).(3)Compared with the model group,the relative expression of Keap1 m RNA in the liver of male and female mice fed high-dose duck oil(2500 mg/kg·BW)decreased by 23.30%and 28.27%(p<0.05),respectively;Nrf 2 m RNA increased by 112.00%and76.92%(p<0.05),respectively;SOD m RNA increased by 68.32%and 70.57%(p<0.05),respectively;GPx m RNA increased by 141.75%and 151.22%(p<0.05),respectively;MT m RNA increased by 56.00%and 49.90%(p<0.05),respectively;Trx m RNA increased by 62.00%and 78.89%(p<0.05),respectively;OGG1 m RNA increased by 34.42%and 28.12%(p<0.05),respectively,MTH1 m RNA increased by40.79%and 43.94%(p<0.05),respectively.There was no significant difference in the relative expression of keap1m RNA,Nrf2m RNA,MT m RNA,Trx m RNA,OGG1 m RNA and MTH1 m RNA in the liver between male and female mice(p>0.05).The relative expressions of SOD m RNA in different groups of liver in mice were significantly different in the normal group,and the relative expression of SOD m RNA in different groups of of liver in mice in the duck oil group after gavage of duck oil was not significantly different(p>0.05).In conclusion:In vitro experiments showed that duck oil has the ability of oxygen free radical absorption,iron reduction,DPPH·scavenging,and can resist the oxidative damage of lipid and DNA.Animal experiments show that duck oil can improve oxidative stress injury induced by injecting mice with D-gal.The mechanism may be achieved by activating Keap1/Nrf2 signal pathway and promoting the transcription level of antioxidant enzymes and antioxidants genes regulated downstream.Besides,there was a certain gender difference in improving oxidative stress injury of mice.