Study On Galactooligosaccharides On The Intestinal Flora Diversity And Repair Of Main Impaired Barrier Function In Mouse

The intestinal flora is closely related to human health and is regarded as an "immune organ" of the human body.The diversity of intestinal flora is closely related to dietary intake.Various oligosaccharides(oligosaccharides)are considered to be intestinal microorganisms.The type and quantity of oligosaccharide intake is very important for the construction of intestinal flora.Modern research shows that the occurrence of various diseases is closely related to the imbalance of intestinal flora.Abuse of penicillin can cause intestinal flora disorders,especially for babies will increase the risk of illness.Galactooligosaccharide(GOS)is a functional oligosaccharide that is allowed to be added to infant formula.Currently,GOS lacks information on the systematic study of the diversity of intestinal flora,especially under the disorder of intestinal flora.GOS has rarely been reported to repair the loss of intestinal barrier function.In this study,C57 BL / 6J mouse were used as the research object,using 16 S r RNA amplicon technology to explore the effects of different doseage of GOS on the diversity of the main intestinal flora of mouse under normal conditions and intestinal disorders.Gas chromatograph was used to detect the main metabolite short-chain fatty acids,an automatic fluorescence microscope was used to observe H & E slices,and inflammatory factors were measured by ELISA to evaluate the damage and repair effects in the intestinal barrier.The main conclusions of this study are as follows:(1)The mouse were fed by three groups of different doseage of GOS with normal drinking water,the experiment showed that:(1)The dominant bacteria in each group were Firmicutes and Bacteroidetes at the phylum level;At 8th weeks,the predominant genus levels in the mid-dosage GOS group were Verrucomicrobiates and Akkermansia.The predominant genus in the high-doseage GOS group were Lachnospiraceae.At 12 th weeks,the dominant bacteria in the mid-doseage GOS group was Lachnospiraceae.The dominant genus of high-dosage GOS was Bifidobateriaceae(2)Mid-doseage and high-doseage GOS group have the most obvious effect on promoting proliferation of Verrucomicrobiates,Akkermansia,Lachnospiraceae and Bifidobateriaceae in mice.(3)The highest levels of acetic acid,propionic acid and butyric acid in the high-dose group.(2)The mouse were fed by three groups of different doseage of GOS with antibiotics in drinking water.The experiment showed that:(1)At the 8th week,the relative abundance of Firmicutes in the intestinal flora of the mice treated with ampicillin sodium at the gate level was higher than that of normal water The feeding group decreased,and the relative abundance of Actinobacteria increased.At 12 th week,the relative abundance of Actinobacteria and Verrucomicrobia in SPM and SPH increased significantly.(2)The relative abundances of Bifidobacterium,Lactobacillus and Akkermansia in the SH group were 5.16%,1.38%,and 0.81%,respectively;the SPH group was 19.79%,13.67%,and 5.68%,respectively.The relative abundances of SPM,SM and Akkermansia are 6.29% and 0.55%,respectively,and the difference between them is significant;(3)The dominant bacteria in the SPM group are Akkermansiaeae,Verrucomicrobiae.The dominant bacteria in the SPH group are Bifidobacteriaceae;(4)High-doseage GOS can promote the production of acetic acid in the intestine,Propionic acid and butyric acid bacteria proliferation.(3)The mouse were fed by three groups of different doseage of GOS with antibiotics in drinking water to explore the effects of different dosesge of GOS in the intestinal barrier and function of mouse with intestinal disorders.The results showed that:(1)GOS increases the proliferation of beneficial bacteria to protect the intestine Dao form,the effect of medium and high-dosage GOS is the most significant.(2)Low-and mid-doseage GOS reduced the level of TNF-? in mice serum(P <0.01)and promoted anti-inflammatory effect,while mid-dasage and high-doseage GOS could significantly reduce the level of LPS(P <0.01).(3)SIg A levels in the low-doseage group were significantly higher than those in the mid-doseage and high-doseage GOS group(P <0.05).The level of albumin in feces increased with the increase of GOS dose(P<0.05),and high-doseage GOS had the best effect.Taken together,mid-doseage GOS has the best effect on alleviating barrier damage in mice with intestinal disorders.