Regulation of posttranslational modifications ofc-fos andc-jun

In an attempt to dissect the signaling pathways involved in transmitting activating signals from the cell surface to the nucleus, the studies described in this thesis examine the regulation of post-translational modifications of the transcription factors c-fos and c-jun in response to signaling pathways. c-fos and c-jun were expressed using the baculovirus/Sf9 insect cell expression system and their DNA-binding and transcription activation abilities characterized in vitro. Results demonstrated that c-fos augments the AP-1-site dependent DNA-binding and transcriptional activation activities of c-jun. In order to examine the regulation of the modifications of c-fos and c-jun, different combinations of the critical signaling proteins the pp60...