| 1.ObjectiveThis paper aims to observe the differences in neuroendocrine function of the normal human pineal gland and the adrenal axis(HPA axis)and the pineal gland,HPA of bronchial asthma patients by observing the different phases of the seasonal season.The dynamic changes of the endocrine function of the axial nerve,and compare and analyze the seasonal characteristics exhibited by the winter phase with other seasons,so as to reveal the biological mechanism of the traditional Chinese medicine "kidney corresponding with winter" from the perspective of the overall regulation of neuroendocrine the basis of learnning.2.Methods2.1 Clinical Trial30 normal people were recruited,including 15 males and 15 females;43 patients with bronchial asthma,including 19 males and 24 females.The staff is fixed and observed for one year in a row.Samples were taken at four points of the spring equinox,summer solstice,autumnal equinox and winter solstice in the year,and blood was taken from the vein and urine samples were taken.2.2Observation Indicators(1)In the one-year spring equinox,summer solstice,autumnal equinox and winter solstice,observe the serum levels of melatonin(MT),corticotropin releasing hormone(CRH)and adrenocorticotropic hormone in normal and bronchial asthma patients.(ACTH),changes in cortisol(COR),adrenaline(E),norepinephrine(NE),and adrenal medulla hormone(ADM)levels.(2)In the one-year spring equinox,summer solstice,autumnal equinox and winter solstice,the changes of plasma aldosterone(ALD)levels in normal and bronchial asthma patients were observed.(3)In the one-year spring equinox,summer solstice,autumnal equinox and winter solstice,observe the changes of urinary 17-hydroxyl(17-COHS)levels in urine of normal people and bronchial asthma patients.3.Result of Clinical Trial3.1 Seasonal changes in serum melatonin levels in normal and bronchial asthma patientsThe levels of serum MT in normal people from high to low in the following season were:winter>autumn>spring>summer,and there were significant differences between winter and summer,the difference was statistically significant(P<0.01);bronchial asthma patients in serum all year round The order of MT from high to low is:spring>autumn>summer>winter,and there is no obvious difference between the four seasons.There was a significant difference between the bronchial asthma patients and the normal human serum in the same phase,and there was a statistically significant difference between the spring and summer(P<0.001).3.2 Seasonal changes in hypothalamic-pituitary-adrenal axis hormone levels in normal and bronchial asthma patients3.2.1 Seasonal changes in serum CRH levels in normal and bronchial asthma patients The levels of serum CRH in normal people from high to low in the following season are:spring>autumn>summer>winter,There is no significant difference in serum CRH levels in normal people throughout the year,but there is a significant decrease in winter compared with the spring,summer and autumn.;the levels of serum CRH in bronchial asthma patients from high to low were:winter>summer>autumn>spring;there was no significant difference in spring,summer and autumn,but there were significant differences compared with winter.There was statistical significance(P<0.05).There was significant difference between the bronchial asthma patients and the normal human serum in the same phase.The difference was statistically significant(P<0.001),The other three seasons serum CRH The average level is lower than normal.3.2.2 Seasonal changes in serum ACTH levels in normal and bronchial asthma patients The levels of ACTH in the serum of normal people from high to low in the following season are:autumn>summer>spring>winter,The levels of ACTH in normal human serum did not change significantly throughout the year,but the winter season was significantly lower than that in spring,summer and autumn;bronchial asthma The levels of ACTH in the serum from high to low in the whole year were:winter>summer>spring>autumn,and there was no significant difference in the four seasons.The difference was not statistically significant.The bronchial asthma patients were in the serum of normal people in the same phase.Compared with the ACTH level,there was a significant difference between the spring and winter seasons,and it was statistically significant(P<0.05).3.2.3 Seasonal changes in serum levels of COR in normal and bronchial asthma patients The levels of COR in the serum from normal to high in the whole year were:autumn>spring>summer>winter,and there were significant differences between autumn and winter,the difference was statistically significant(P<0.001),There is a significant decrease in winter compared with the spring,summer and autumn.;The levels of COR in the serum from high to low were:winter>summer>spring>autumn,and there were significant differences between spring and autumn and winter,and there was statistical significance(P<0.05).Patients with bronchial asthma and the same phase There was a significant difference in the level of COR in normal human serum compared with winter,and it was statistically significant(P<0.001)).3.2.4 Seasonal changes in plasma ALD levels in normal and bronchial asthma patients In normal people,the ALD levels in plasma from high to low in the following season are:autumn>winter>summer>spring,there is no obvious change trend in the four seasons;the ALD level in plasma of patients with bronchial asthma is from high to low in the following season:winter>summer>Spring>Autumn,there was no obvious change trend in the four seasons;there was significant difference in the ALD level between the bronchial asthma patients and the normal people in the same phase,and there was statistical significance(P<0.001).3.2.5 Seasonal changes in 1 7-OHCS levels in urine of normal and bronchial asthma patients The level of 17-OHCS in the urine of normal people from high to low in the following season is:autumn>spring>summer>winter,there is no obvious change trend in the four seasons;the level of 17-OHCS in the urine of patients with bronchial asthma is high to low throughout the year.In the following order:winter>spring>autumn>summer,there was no obvious change trend in the four seasons;there was no significant difference in the urinary asthma patients compared with the 17-OHCS level in the urine of normal people in the same phase.3.3 Seasonal changes of adrenal medulla hormone levels in normal and bronchial asthma patients3.3.1 Seasonal changes in serum NE levels in normal and bronchial asthma patientsThe level of NE in serum from normal to high in the whole year was:spring>autumn>summer>winter,and there was significant difference between spring and winter,the difference was statistically significant(P<0.001);one year for bronchial asthma patients The levels of E in the serum of the four seasons were as follows:winter>summer>spring>autumn,the spring,summer and autumn three seasons were significantly different from the winter,and statistically significant(P<0.001);bronchial asthma patients and the same phase The level of E in normal human serum was significantly different from that in autumn,and it was statistically significant(P<0.05).3.3.2 Seasonal changes in serum E levels in normal and bronchial asthma patientsThe levels of serum E in normal people from high to low in the following season are:spring>autumn>summer>winter,in which there is a significant difference between spring and autumn and winter,the difference is statistically significant(P<0.05);The level of serum E in the four seasons from high to low is:winter>summer>spring>autumn,which has significant difference between spring and autumn and winter,and has statistical significance(P<0.05);bronchial asthma patients and the same phase There was a significant difference in the E level in the normal human serum compared with the winter,and it was statistically significant(P<0.001).3.3.3 Seasonal changes in serum ADM levels in normal and bronchial asthma patientsThe levels of ADM in the serum of normal people from high to low in the following season are:autumn>spring>summer>winter,and there are significant differences between spring and autumn and winter,the difference is statistically significant(P<0.001);The levels of ADM in serum from high to low were:winter>summer>spring>autumn,and there were significant differences between spring and autumn and winter,and statistically significant(P<0.001);patients with bronchial asthma and the same phase There was a significant difference in ADM levels in normal human serum compared with spring and autumn,and it was statistically significant(P<0.05).4.Conclusion(1)The neuroendocrine-related hormone secreted by MT and HPA axis in normal humans has obvious seasonal rhythm,among which MT has the highest level in winter,while HAP axis related hormone has the lowest level in winter,and MT has seasonal regulation effect on HPA axis.And the most obvious performance in winter,the regulation of the two can further reveal the scientific significance of "kidney corresponding with the winter,kidneys governing storage".(2)Compared with normal people,the level of MT in the bronchial asthma patients did not change significantly throughout the year,and the seasonal rhythm changed abnormally.The HPA axis neuroendocrine-related hormone also showed obvious seasonal rhythm disorder,and the two were synchronous.It can be seen that the seasonal rhythm disorder of melatonin in the pineal gland of bronchial asthma patients affects the seasonal rhythm of neuroendocrine factors of HPA axis,and the ability of seasonal regulation of neuroendocrine of HPA axis in bronchial asthma patients is weaker than that of normal people.The results of this study showed that HPA axis secretion hormone in bronchial asthma patients increased significantly in winter,combined with the physiological effects of HPA axis hormones,indicating that bronchial asthma patients have winter kidney loss,fine gas leakage,and hyperactivity.On the one hand,the results of this study can reveal the intrinsic mechanism of increased incidence of bronchial asthma in winter,and on the other hand,the biological mechanism of traditional Chinese medicine " kidney corresponding with the winter,kidney main gas" is explained from the perspective of neuroendocrine. |
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