| BackgroundPseudomonas aeruginosa is a conditional pathogenic bacteria with strong resistance gene and clonal transmission ability.It is the main nosocomial pathogenic bacteria causing pneumonia in hospitalized patients.In recent years,the incidence of infections caused by Pseudomonas aeruginosa has been high,the condition is critical,the treatment is difficult,and the mortality rate is high.However,the resistance of Paeruginosa to various antibiotics has also generally increased.The resistance mechanism of spores and strengthening the monitoring of resistance to Pseudomonas aeruginosa are of great significance for the treatment of Pseudomonas aeruginosa pneumonia.The treatment of modern medicine is mainly to improve the symptoms of Pseudomonas aeruginosa pneumonia.Broad-spectrum antibiotics are widely used,which also leads to the resistance of Paeruginosa to multiple antibiotics.Motherland Medicine has its unique advantages in the prevention and treatment of Pseudomonas aeruginosa pneumonia,and has achieved significant clinical efficacy.It can not only effectively improve the clinical symptoms of patients,but also improve immunity,improve quality of life and delay survival time.Therefore,it is necessary to further explore the mechanism of action of traditional Chinese medicine in treating Pseudomonas aeruginosa infection.ObjectiveObserve the evaluation of Fuzheng Jiedu Huayu Decoction on the blood routine indexes,lung tissue pathology and curative effect of chronic infection caused by MDRPA and explore the mechanism of Fuzheng Jiedu Huayu Decoction in the treatment of chronic MDRPA pneumonia Based on the IL-36/NFκB.and explore the possible mechanism of Fuzheng Jiedu Huayu Decoction through IL-36 treatment of chronic MDRPA pneumonia.Method120 rats were randomly divided into 5 groups(blank group,model group,Chinese medicine group,western medicine group and Chinese and Western group)for one week of adaptive feeding,and an MDRPA chronic pneumonia rat model was established by oral tracheal intubation method.Give twice a day.The Chinese medicine group was given intragastric injection of Fuzheng Jiedu Huayu Granules 2ml/200g,the western medicine group was injected intraperitoneally with piperacillin sodium tazobactam sodium solution 0.36ml/200g,and the Chinese and Western groups were given Fuzheng Jiedu Huayu granules Stomach and injection were intraperitoneally injected with piperacillin sodium and tazobactam sodium solution.The blank group and model group were given intragastric administration with equal amount of sterile water every day.Rats were free to eat and drink water.After modeling,observe the general condition of the rats.The rats were sacrificed 3 days,7 days,14 days,and 21 days after modeling.Blood was taken from the abdominal aorta to determine blood routine.Serum was used for enzyme-linked immunosorbent assay.The lung tissues of rats were collected and stained with HE.The degree of lung tissue damage was analyzed under light microscope.The lung homogenate was used for bacterial culture.The upper lobe of the right lung was taken for Western blotting to detect the expression of P65 protein.ResultsExperiment One:MDRPA chronic pneumonia rat model was successfully observed through lung tissue pathology observation and lung homogenate count.The results of blood routine inflammatory indicators suggested that Fuzheng Jiedu Huayu Decoction can alleviate inflammation in model rats to a certain extent.The symptoms and signs of the rats in the Chinese medicine group was significantly improved.Taken together,Fuzheng Jiedu Huayu Decoction plays a therapeutic role in improving the general condition of chronic pneumonia in rats.Experiment Two:At the cytokine level,comparing with the blank group,the TNF-α in the model group increased significantly,with a significant difference(P<0.05);compared with the model group,the TNF-α in the traditional Chinese medicine group was in the model 7,14,There was a significant difference between the 21-day group(P<0.05),the western medicine group had a significant difference in the model 7 and 21-day group(P<0.05),and the Chinese and Western groups had a significant difference in the model 14 and 21-day group(P<0.05)0.05).Compared with the blank group,the IL-36 of the model group was significantly increased,and there was a significant difference between the 3 and 21 days of modeling(P<0.05);compared with the model group,the traditional Chinese medicine group was 7,14,and 21 days of modeling There was a significant difference between the groups(P<0.01),IL-36 in the Western medicine group had a significant difference in the 3 and 7-day modeling groups(P<0.01),and IL-36 in the Chinese and Western groups had a significant difference in the 3 and 21-day modeling groups Difference(P<0.05).Compared with the blank group,the LL-6 of the model group increased significantly,and there was a significant difference between the 3 and 21 days of modeling(P<0.05);compared with the model group,the IL-6 of the traditional Chinese medicine group was in the modeling of 7,14 The 21-day group had a significant difference(P<0.05),and the Western medicine group had a significant difference between the 3 and 7-day modeling groups(P<0.05).Compared with the blank group,IL-10 in the traditional Chinese medicine group increased significantly on the 3rd,14th,and 21st day of modeling,and there was a significant difference(P<0.05).Significant difference(P<0.05);compared with the model group,IL-10 in the traditional Chinese medicine group and the western medicine group increased significantly in the 14-day modeling group,with a significant difference(P<0.05).Correlation analysis results showed that IL-36 and TNF-αshowed statistical differences in the 3-day group,7-day group and 21-day,group,and IL-36 and IL-6 showed statistics in the 7-day group and 21-day group difference.Consider that Fuzheng Jiedu Huayu Recipe may reduce the production of pro-inflammatory cytokines IL-36,TNF-α and IL-6 by inhibiting the NF-κB transcription factor and up-regulate the expression of anti-inflammatory cytokine IL-10,thereby reducing inflammatory Damage to the lungs.At the protein expression level,comparing with the blank group,the model group had a significant increase in NF-κB subunit P65,with a significant difference(P<0.05),and the Western medicine group had a significant increase in the model 3 and 21 days of modeling,with a significant difference(P<0.05).Compared with the model group,the Chinese medicine group and the Chinese and Western groups increased signi:ficantly with a significant difference(P<0.01)on the 3rd day of modeling.Compared with the model group,NF-κB subunit P65 increased significantly with a significant difference on the 3rd and 21st day of modeling Difference(P<0.05).It is considered that Fuzheng Jiedu Huayu Decoction may reduce the expression of NF-κB subunit P65 and reduce the active expression of inflammatory cytokines by inhibiting the NF-κB transcription factor,and finally play a protective role in rat lung tissue.Conclusion1.Fuzheng Jiedu Huayu Decoction has a positive therapeutic effect on the general condition of model rats and the pathology of lung tissue under light microscope,and can reduce the degree of lung inflammation;2.Fuzheng Jiedu Huayu Decoction Fuzheng Jiedu Huayu Recipe may exert its therapeutic effect on chronic pneumonia of MDRPA by inhibiting IL-36/NFκB and affecting the expression of NF-κB subunit P65 protein and inflammatory cytokines. |
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