The Effect Of Xinnaomaikang On Blood Lipids And Serum Inflammatory Factors In Atherosclerotic Rabbit Model

Purpose: To observe the effects of Xinnaomaikang Fang on total cholesterol(TC),triglyceride(TG),low density lipoprotein(LDL),high density lipoprotein(HDL),and C-reactive protein(CRP)in atherosclerotic rabbit models,human monocyte chemotactic protein-1(MCP-1),intercellular adhesion molecule-1(ICAM-1)levels and common carotid artery tissue morphology,and explore its anti-atherosclerotic mechanism.Material and method: After 7 days of adaptive feeding of male Japanese big-eared white rabbits,they were randomly divided into a blank group and a model group.The atherosclerotic animal model group was modeled by balloon surgery and high-fat diet.Modeled animals were randomly divided into model group,Xinnaomaikang clinical equivalent dose group,Xinnaomaikang clinical double dose equivalent group,and traditional Chinese medicine positive control Xuezhikang group.Intragastric administration started on the 2nd day after the modeling operation.The blank group and the model group were given the same amount of distilled water,and the other groups were treated with the corresponding doses of drugs once a day for 4 weeks.After the administration,the white rabbits were sacrificed and the following indicators were measured:(1)TC,TG,LDL,HDL,Hcy,CRP levels;(2)serum MMP-9,MCP-1,ICAM-1 Level;(3)Observe the pathomorphological changes of common carotid artery HE staining.Results:1.Model evaluation: TC,TG,LDL,and HDL in the white rabbits of the model group were significantly higher than those in the blank group(P < 0.05).Combined with the pathological morphological results of the white rabbits in the model group after HE staining,the experiments successfully established the hyperlipidemia and atheroma models.2.Changes of blood lipid indexes: compared with the model group,the contents of TC,TG an d LDL in xinnaomaikang clinical equivalent dose group and xinnaomaikang twice clinical eq uivalent dose group decreased significantly(P < 0.05),and the contents of HDL increased sig nificantly(P > 0.05).3.Changes of serum inflammatory factors: compared with the blank group,the levels of CRP,MMP-9,MCP-1 and ICAM-1 were significantly increased in the model group;compared with the model group,the levels of CRP,MMP-9 and ICAM-1 were significantly decreased in the xinnaomaikang clinical equivalent dose group and xinnaomaikang twice clinical equivalent dose group(P < 0.05).There was no significant difference in MCP-1 level between xinnaomaikang clinical equivalent dose group and xinnaomaikang twice clinical equivalent dose group and model group(P > 0.05).4.Pathological changes of the common carotid artery: the intima of the common carotid artery in the blank group was complete,smooth,uniform in thickness,without significant proliferation,and the thickness of the smooth muscle layer was uniform;the structure of the inner wall of the artery in the model group was unclear,and the intima was significantly increased due to plaque Thick and uneven thickness,there are a large number of foam cells and a small amount of cell debris deposited under the intima,and the shape is irregular;a small number of plaques were seen in the Xuezhikang group,the intima was thickened,and foam cells were deposited underneath,and a small amount of inflammatory cells infiltrated.The membrane is intact.Xinnaomaikang clinical equivalent dose group showed atheromatous plaques,vascular intima was not smooth,part of the intimal structure thickened and prominent,protruding to the arterial lumen,and a small amount of foam cells and inflammatory cells were found in the plaque.A small amount of atherosclerotic plaques were seen in the Xindoumaikang clinical double-equivalent dose group,the lining of the blood vessels was not smooth,there was no obvious thickening and bulging,and the deposition and infiltration of foam cells and inflammatory cells under the endometrium were reduced.Conclusion:1.Xinnaomaikang can improve the lipid infiltration of carotid artery intima by regulating blood lipid,so as to play an anti atherosclerotic role.2.Xinnaomaikang can significantly reduce the levels of CRP,MMP-9 and ICAM-1 in the model animals,but also has the trend of reducing the level of MCP-1,which proves that it can play an anti atherosclerotic role by regulating inflammatory factors.