An Experimental Study On The Effect Of GP17 On The Expression Of HO-1, NQO1, And GST In Oxidative Stress Of Skin Photoaging Mice

Purpose:In this study,the skin photoaging of mice was induced by ultraviolet(UV)radiation.The effects of oxidative stress on the expression of HO-1,NQ01,GST,and Gypenoside XVII(GP-17)as an intervention factor to explore the effect of Gypenoside XVII on UV-induced skin photo-aging mice Excitement mechanism.Material and method:Sixty healthy male Kun Ming mice were randomly divided into six groups: control group,UV model group,high-dose Gypenoside XVII group,medium-dose Gypenoside XVII group,low dose Gypenoside XVII group and VE group,ten in each group.Mice normal feeding one week to adapt to the environment,the mouse back hair shaving about 2×2cm2 area,exposing the back of the skin,once every three days to shave a hair to ensure that the back of the mouse skin exposure,except for the blank control group,the remaining five were 40 W ultraviolet light(UVA+ UVB)under the irradiation,a total of twelve weeks of irradiation,irradiation from the first two weeks,2-5 weeks,six days a week irradiation,0.5h daily exposure,6-9 weeks,weekly irradiation Six days,daily exposure to 1h,the first 10-13 weeks,six days a week irradiation,daily irradiation 2h,to the end of 13 weeks,ending UV irradiation,UV irradiation intensity accumulated 224.427 J / cm2.At the beginning of the fourteenth week,mice in control group and mice in the UV model group were given Normal Saline,high-dose Gypenoside XVII group,middle-dose Gypenoside XVII group,low-dose Gypenoside XVII group,and VE group were given high-dose aescin Gypenoside XVII,mid-dose Gypenoside XVII,low-dose Gypenoside XVII and gastric lavage VE olive oil,dosing time once daily for two weeks.Mice were sacrificed on the 16 th week,the skin area of about 1×1cm2 in the back of the mice,remove subcutaneous fat,and was frozen in-70 ℃ refrigerator to be tested.Detection method:Western-Blot method was used to detect the expression of HO-1,NQ01 and GST in skin tissue.Results: 1.Mouse skin tissue antioxidant protein HO-1 expression results The results of the expression of antioxidant protease HO-1 in the skin tissue of mice with photodamage were shown:The protein expression of HO-1 in the blank control group was lower than that in the control group(P<0.05).Compared with the UV-irradiated UV model group,the expression of HO-1 was slightly elevated(P<0.05),While the high-dose Gypenoside XVII group,aseptic mid-dose Gypenoside XVII group,low-dose Gypenoside XVII group and the VE group compared with the blank control group,the expression of antioxidant protein HO-1 increased significantly,The difference was significant(P <0.01).Compared with the UV model group,the expression of the antioxidant enzyme HO-1 in high-dose Gypenoside XVII groupand mid-dose Gypenoside XVII group and VE group(P <0.01)(P<0.05).Compared with the high-dose Gypenoside XVII group and middle-dose Gypenoside XVII group,the low dose Gypenoside XVII group(P<0.01)(P <0.05).In the high-dose Gypenoside XVII group,middle-dose Gypenoside XVII group,low-dose Gypenoside XVII groupand VE group,the most obvious increase of HO-1 expression was high dose Gypenoside XVII group.2.Mouse skin tissue antioxidant protein NQO1 expression results The results of the expression of antioxidant protease NQO1 in the skin tissue of mice with photodamage were shown: the expression of NQO1 the control group was lower,the UV group,the high-dose Gypenoside XVII group,the medium-dose Gypenoside XVII group,the low-dose Gypenoside XVII group and VE group with the blank control group,the expression of antioxidant protein NQO1 increased significantly,the difference was significant(P <0.01).Compared with the UV model group,the expression of protease NQO1 increased more obviously in the high dose Gypenoside XVII group,the middle dose Gypenoside XVII group and VE group were significantly higher than the model group,the difference was statistically significant(P <0.01)(P<0.05).Compared with the high-dose and mid-dose Gypenoside XVII group,the NQO1 expression content in the low-dose Gypenoside XVII group although increased,but not as obvious,the difference was statistically significant(P <0.01)(P<0.05).In the high-dose Gypenoside XVII group,middle-dose Gypenoside XVII group,low-dose Gypenoside XVII groupand VE group,the most obvious increase of NQO1 expression was high dose Gypenoside XVII group.3.Mouse skin tissue antioxidant protein GSTexpression results The results of the expression of antioxidant protease GST in the skin tissue of mice with photodamage were shown:the expression of GST the control group was lower,the UV group,the high-dose Gypenoside XVII group,the medium-dose Gypenoside XVII group,the low-dose Gypenoside XVII group and VE group with the blank control group,the expression of antioxidant protein GST increased significantly,the difference was significant(P <0.01).Compared with the UV model group,the expression of anti-oxidase GST in the high-dose Gypenoside XVII group and the middle dose Gypenoside XVII group increased obviously,the difference was statistically significant(P<0.01);Compared with the Gypenoside XVII group,the expression of anti-oxidase GST in middle dose Gypenoside XVII group,low dose Gypenoside XVII groupand VE group had no significant difference(P <0.05)(P <0.01);Compared with the medium dose Gypenoside XVII group,the expression of anti-oxidase GST in low dose Gypenoside XVII group and VE group was less,the difference was statistically significant(P<0.01)(P<0.05).In the high-dose Gypenoside XVII group,middle-dose Gypenoside XVII group,low-dose Gypenoside XVII group and VE group,the most obvious increase of GST expression was high dose Gypenoside XVII group.Conclusion: 1.UV irradiation can cause oxidative stress in mice,which can turn on the defense mechanism of anti-oxidative damage by inducing the expression of auto-antioxidant proteases HO-1,NQO1 and GST downstream of the signal transduction pathway.2.Photosensitive mice secrete HO-1,NQO1,GST content is not enough to resist oxidative stress injury,Gypenoside XVII can enhance the expression of HO-1,NQO1,GST,effectively inhibit oxidative damage,against the skin Light aging,and high-dose group the best.