| Background:Lung cancer is currently the leading malignant tumor with the highest morbidity and mortality worldwide.Because the early symptoms of lung cancer are not obvious,most of them are found in the middle and late stages,and the chance of surgical resection has been lost.The benefits of traditional radiotherapy and chemotherapy are very limited.The emerging targeted therapy and immunotherapy are selective,and minimally invasive ablation Because of its small trauma,definite curative effect,simple operation,high safety,wide application population and high reproducibility of surgery,it has gradually become a new model for treating lung cancer.Traditional Chinese medicine believes that local metabolites with strong local metabolism,rapid growth,easy to walk,and depletion of qi and blood can be distinguished as "yang syndrome",while local metabolism is relatively slow,growth rate is slow,and it is difficult to metastasize.The cancer can be identified as "negative evidence."Hot people are cold,cold is hot"is one of the basic responsibilities of traditional Chinese medicine.Therefore,the malignant tumors that are identified as "yang syndrome"should be treated with "cold",and the curative effect may be more significant.Clinically,cold ablation represented by cryoablation of argon-helium cryoablation and thermal ablation represented by radiofrequency ablation,microwave ablation and laser ablation are widely used in the treatment of lung cancer.Cold ablation and thermal ablation reduce local tumor burden of lung cancer.The curative effect is clear,but the efficacy of the two is not conclusive Secondly,some clinical studies and basic research suggest that cold ablation and thermal ablation can stimulate subsequent immune responses,but the difference between the two is still inconclusive.In theory,different treatment methods with different cold and heat attributes are used to treat local syndrome differentiation as "yang syndrome".In the case of a malignant tumor,the immune response that causes the body may be different.Therefore,the efficacy of cryoablation and thermal ablation and its related mechanisms of action are of great significance for subsequent clinical application and basic researchObjective:1.To determine the difference in curative effect between cryoablation,thermal ablation and metal knife in the treatment of LLC lung cancer-bearing mice;2.To determine the effects of three treatments:ablation,thermal ablation and metal knife on the immune function of LLC lung cancer-bearing mice.And preliminary exploration of relevant mechanisms.Methods:1.To determine the difference in efficacy of cryoablation,thermal ablation and metal knife in the treatment of Lewis lung cancer-bearing mice:40 mice with uniform tumor size were randomly divided into cryoablation group,thermal ablation group,surgical resection group and incision.Four groups in the suture group,10 in each group,were subjected to cryoablation,thermal ablation,surgical resection,incision and suture treatment,and observed the survival,activity and hair condition of the mice;2.Cryoablation and thermal ablation for Lewis lung cancer The effect of immune function in tumor-bearing mice and its related mechanisms:40 mice with uniform tumor size were randomly divided into cryoablation group,thermal ablation group,surgical resection group and incision suture group,with 10 rats in each group.Cryoablation,thermal ablation,surgical resection,and incision suture were performed separately.Three groups of mice were sacrificed on the 3rd,7th and 10th day after intervention,and the peripheral blood samples of the mice were taken through the posterior orbital vein.The T cell subsets in the peripheral blood of the tumor-bearing mice were detected by flow cytometry.(CD3+,CD4+,CD8+);exfoliated mouse tumor tissue,weighed,calculated tumor inhibition rate,fixed with paraformaldehyde,routinely made sections,and detected tumor lesions IL-2,IL-6,TNF-α by immunohistochemistry Expression of cytokines;spleen tissue of mice was excised,weighed,and spleen index was calculated;lung tissue of mice was exfoliated,and tumor metastasis of mice was observed.Results:1.The survival time of LLC lung cancer-bearing mice after cryoablation,thermal ablation,metal knife and incision suture was 18.67±2.80 days,18.17±4.62 days,17.67±3.33 days,18.50±2.74 days,respectively.There was no statistically significant difference between the groups(P>0.05)Results:1.After cryoablation,thermal ablation,surgical resection,incision and suture intervention in Lewis lung cancer-bearing mice,the survival time of cryoablation,thermal ablation and surgical resection group was longer than that of incision suture group,but the difference between groups was not statistical.significance2.horizontal comparison:a,7days after surgery,T cell subsets,CD4/CD8 ratio cryoablation group was the highest,the difference between the groups was statistically significant(F =6.426,P = 0.016);spleen weight,The incision group was the highest,followed by the cryoablation group and the thermal ablation group.The surgical resection group was the lowest,and the difference between the groups was statistically significant(F=7.267,P=0.011).In the IL-6 area,the cryoablation group was the highest,followed by the highest.The surgical resection group and the incision suture group were the lowest in the thermal ablation group,and the difference between the groups was statistically significant(P=0.012).b.In the 10 days after operation,the percentage of CD3 cells in the cryoablation group was the highest,and the difference between the groups was statistically significant(P=0.013).The spleen weight was the highest in the thermal ablation group,followed by the incision suture group and the cryoablation group.The surgical resection group was the lowest,the difference between the groups was statistically significant(F=4.674,P=0.024);the spleen index was the highest in the open suture group,followed by the thermal ablation group,the cryoablation group,and the lowest in the surgical resection group.Statistically significant(P=0.030);In the IL-2 group,the cryoablation group was the highest,followed by the incision suture group and the surgical resection group.The thermal ablation group was the lowest,and the difference between the groups was statistically significant(P=0.024).In the TNF-a group,the surgical resection group was the highest,followed by the highest.The cryoablation group and the incision suture group were the lowest in the thermal ablation group,and the difference between the groups was statistically significant(P=0.015)3.longitudinal comparison:a,cryoablation group at different time periods,the percentage of CD8 cells was the highest after 3 days,decreased 7 days after surgery,and increased again 10 days after surgery,the difference was statistically significant at different time points(P=0.043)The spleen weight and spleen index increased with time,and the spleen weight was statistically different at different time points(F=10.070,P=0.009);IL-6 increased first and then decreased with time,different The time difference was statistically significant(P=0.000);TNF-a increased with time,and the difference was statistically significant at different time points(P=0.043).b.The percentages of CD3 cells and CD4 cells increased first and then decreased with time in the thermal ablation group.The percentage of CD3 cells in different time periods was statistically significant(P=0.031);the spleen weight and spleen of mice The index increased with time,and the difference of spleen weight was statistically significant(F=11.532,P=0.009);IL-6 increased with time,and the difference was statistically different at different time points(P=0.024);c,the percentage of CD4 cells increased first and then decreased with time in the incision group.The percentage of CD4 cells in different time periods was statistically significant(F=4.826,P=0.048).The percentage of CD8 cells decreased with time,and the difference was statistically significant at different time points(P=0.035).The spleen weight and spleen index of mice increased with time,and the difference of spleen weight was statistically significant.(F=11.532,P=0.009),the difference in spleen index was statistically significant(F=9.955,P=0.009).d.The percentages of CD3 cells,CD4 cells and CD8 cells increased first and then decreased with time in the surgical resection group.The percentage of CD3 cells in different time periods was statistically significant(P=0.017);CD4/CD8 ratio The difference was statistically significant with time(P=0.048);IL-6 increased with time,and the difference was statistically significant(P=0.012);Conclusions:1.There is no significant difference in the efficacy of cryoablation,thermal ablation and surgical resection for Lewis lung cancer-bearing mice2.cryoablation,thermal ablation,surgical resection three intervention methods in a short period of time on the T cell subsets of LLC tumor-bearing mice,there is no significant difference,but with the change of time,late cryoablation of LLC tumor-bearing mice The enhancement of immune function is superior to thermal ablation and surgical resection3.cryoablation,thermal ablation,surgical resection three intervention methods in a short period of time on the IL-2,IL-6,TNF-a in LLC tumor-bearing mice,there is no significant difference,but with the change of time,late cryoablation was superior to thermal ablation and surgical resection in the promotion of IL-2 and IL-6 secretion in LLC tumor-bearing mice4.cryoablation can promote the secretion of IL-6 and TNF-a in LLC tumor-bearing mice,and thermal ablation can promote the secretion of IL-6 in LLC tumor-bearing mice. |
PDF Full Text Request