Preparation And Function Study Of HEV Protective Antibody

Objective:To reseach whether the monoclonal antibodies in mice based on genetic Immunization of HEV neutralization epitope(p179)could induce neutralizing antibody response.Methods:The neutralization epitope HEV-pl79 coding gene was subcloned into a eukaryotic expression vetor venus to construct recombinant plasmind Venus-C1-179.BALB/c mice was immuned with tail intravenous injection method,Detecting the level of antibody response of mice after Immunization by ELISA,Human HEV cell infection model by C3A cell line,Detection of nenutralizing activity of protective antibody after neutralization test by RT-PCR.Results:Antibodies to hepatitis E virus neutralizing epitope p179 of 6 strains were prepared,the antibody 2G89F9 can effectively terminate HEV virus,demonstrated the antibody could effectively prevent HEV infecting susceptible cells C3A,and equipped with neutralizing activity against HEV infection.Conclusion:We found that the monoclonal antibodies in mice based on genetic Immunization of HEV neutralization epitope(p179)could induce neutralizing antibody immune response.