The Effect Of Exosomes Secreted By T Cells Caused By Radiation On The Migration And Invasion Of Human Esophageal Squamous Cell Carcinoma Cells And Its Mechanism

[Objective]Exosomes are nanocapsules derived from normal cells and tumors that can be detected in human biological fluids such as cell culture supernatants and plasma.The characteristics of the exosomes come from the "normal" cells are not clear.Although many studies have investigated exosomes of hematopoietic cells,few have known exosomes from T cells,although these cells play an important role in congenital and acquired immunity.The aim of this study was to investigate the effect of exosomes secreted by irradiated T cells on human esophageal squamous cell carcinoma cell line TE13.[Materials and methods]A cck-8 assay was used to detect the ability of exosomes to inhibit the proliferation of TE13 cells.The effects of exosomes on the migration and invasion of TE13 cells were investigated by in vitro invasion and wound healing.Western blot analysis was performed to study the effect of exosomes on the expression of β-catenin snail and NF-κB,all of which were associated with EMT.[Results]The study showed that exosomes suppress the proliferation but accelerate the metastasis of TE13 cells in a time-and dose-dependent manner.In addition,exosomesobviously increased the expression of NF-κB,snail and beta-catenin in TE13 cells.[Conclusion]The results of this study indicate the important role of exosomes from T cells in the progression of esophageal cancer:exosomes derived from T cells promote metastasis of esophageal cancer,May be mediated by upregulatzng β-catenin and NF-κB/snail pathways.Besides,the study supports the use of exosomes as a nearly perfect example of biomimetic nanocapsules that can be used in future strategies for the treatment of various diseases,including cancer.