The Effect Of Resveratrol Albumin Nanoparticles On The Proliferation And Apoptosis Of Human Gallbladder Cancer GBC-SD Cells

Objective To prepare the RES-BSANP,and to explore the effect and mechanism research of the proliferation and apoptosis for human cholecystic cell line GBC-SD in vitro.Provides new way for RES’ clinical application and cancer clinical care.Method The encapsulation efficiency and drug loading of albumin were determined by HPLC method.The RES and RES-BSANP were measured by CCK-8 method.And the effect of RES and RES-BSANP on the expression of Caspase-3,Caspase-8,Caspase-9 were detected by polymerase chain reaction(RT-PCR).Results(1)Uses desolation the Chemical cross-linking to prepare RES-BSANP,will get the average drug loading efficiency is 5.97% and average encapsulation efficiency is 37.4%.(2)Compared with RES,5-Fu and RES-BSANP inhibited the proliferation of gallbladder carcinoma GBC-SD was significantly enhanced in 20~80 μg/ml concentration,and showed concentration dependent on the 10~80 μg/ml in the range of concentration.(3)5-Fu,RES and albumin nanoparticles can increase the relative expression of Caspase-3,Caspase-8,Caspase-9 mRNA in gallbladder carcinoma cell line GBC-SD.Which indicates that the three can affect the Caspase signal transduction pathway,activation of Caspase-8 and Caspase-9 respectively into exogenous and endogenous apoptosis,stimulate Caspase cascade,activation of downstream effectors in common Caspase-3,thereby promoting apoptosis of gallbladder cancer cells,the inhibition of cell proliferation of gallbladder carcinoma.Conclusion RES-BSANP have a significant inhibitory effect on the proliferation of gallbladder carcinoma GBC-SD cells,and the inhibitory effect is stronger than that of RES alone.This mechanism may be related to up-regulation of Caspase gene expression,leading to apoptosis.