Research On The Construction Of Keratin Composite Fibrovascular Tissue Engineering Scaffold

Tissue-engineered blood vessels(TEBVs)have extensive and important applications in blood vessel replacement therapies.Fast and stable endothelialization is recognized as an effective approach to prevent intraluminal thrombosis and intimal hyperplasia caused by restenosis and other issues.Keratins are excellent non-antigenic materials with good biocompatibility,biodegradability and no immunogenicity.However,the blood clotting nature limits their biomedcial applications.In this work,human hair keratins(HHKs)were first extracted by reduction method,and keratin derivatives were then prepared by various chemical methods to improve blood compatibility.Finally,keratin derivatives were coelectrospun with biodegradable poly(?-caprolactone)(PCL)to prepare vascular tissue engineering scaffolds.(1)Heparinized the coelectrospun PCL/keratin mats for vascular tissue engineeringPCL/keratin nanofibrous scaffolds were first prepared by electrospinning and then heparinized to improve blood compatibility.The mats were characterized by SEM,ATR-FTIR,and XPS.These mats were proved to have good cytocompatibility and blood compatibility.Furthermore,the heparinized PCL/keratin mats had the potential to catalyze endogenous nitric oxide(NO)donor S-nitrosoglutathione(GSNO)to release NO,which was expected to be used as vascular tissue engineering scaffold.(2)Coelectrospinning of PCL/sulfated keratin mats for vascular tissue engineering.Sulfated keratin was coelectrospun with PCL to produce PCL/sulfated-keratin mats.The mats were characterized by Elemental Analysis,FTIR,SEM,and XPS.These mats were proved to have good cytocompatibility and blood compatibility.Furthermore,PCL/sulfated-keratin mats had the potential to catalyze endogenous NO donor GSNO to release NO,which was expected to be used as vascular tissue engineering scaffold.(3)Coelectrospinning of poly(?-caprolactone)/phosphobetainized keratin mats for vascular tissue engineering.Keratin modified with MPC(phosphobetaine)was coelectrospun with PCL to produce PCL/phosphobetainized keratin mats.The mats were characterized by Elemental Analysis,FTIR,SEM,and XPS.These mats were cytocompatible and blood compatible.Furthermore,PCL/phosphobetainized keratin mats had the potential to catalyze endogenous NO donor GSNO to release NO,which was expected to be used as vascular tissue engineering scaffold.(4)Coelectrospinning of PCL/S-nitrosated keratin mats for vascular tissue engineering.S-nitrosated keratin(KSNO)was synthesized and coelectrospun with PCL to produce PCL/KSNO mats.The mats were characterized by UV-Vis,SEM,and XPS.These mats were proved to have good cytocompatibility and blood compatibility.Furthermore,PCL/KSNO mats could release NO with ascorbic acid catalyst,which was expected to be used as vascular tissue engineering scaffold.