| Objective:Based on the previous related research,we established a Lewis lung cancer mouse model to investigate the anti-tumor effect of Feiyanning on Lewis lung cancer mice and the regulation mechanism of Feiyanning on mitochondrial division and fusion signals in transplanted tumor tissues.Methods:Thirty C57BL/6 mice were randomly divided into model group,paclitaxel group and Feiyanning group,and a subcutaneous xenograft model of Lewis lung cancer mice was constructed.After successful modeling,the model group was given(normal saline gavage;intraperitoneal injection of normal saline),paclitaxel group(normal saline gavage;paclitaxel intraperitoneal injection),Feiyanning group(Feiyanning stomach;intraperitoneal injection of normal saline)intervention,and observe the survival state,body weight changes,transplanted tumor volume changes.The mice were sacrificed and the tumor tissues were taken.The tumor volume and tumor inhibition rate of the three groups were compared.The mitochondrial morphology of the transplanted tumor tissues of the three groups was observed under electron microscope.The transplanted tumors were detected by Real-time PCR and immunohistochemistry.Tissue mitochondrial division(Drp1,Fis1),fusion(OPA1,MFN1,MFN2)related signal m RNA and protein expression differences.Results:1.General situation: After intervention,compared with the model group and paclitaxel group,the mice in the Feiyanning group showed better survival status.Compared with the model group,the weight of the paclitaxel group was increased slower,the difference was statistically significant(P<0.05).Compared with the Feiyanning group,the weight of the paclitaxel group was increased slower(P<0.01).Compared with the model group,the weight of the lung Feiyanning group increased rapidly,but the difference was not statistically significant(P>0.05).2.The tumor inhibition rate was 29.77% in the paclitaxel group and 33.52% in the Feiyanning group.Compared with the model group,the two groups had significant differences(P<0.01),but the difference between the two groups were no statistically significant(P>0.05).3.Electron microscopy: The mitochondrial electron microscopy of the model group was mostly elliptical,less elongated,and strip-like.Compared with the model group,the mitochondrial morphology of the paclitaxel group showed small round and small oval shapes,and very few long strips.The mitochondria of the Feiyanning group are mostly long-shaped,strip-like,with less round and elliptical shapes.4.Level of m RNA expression:(1)Compared with the model group,the expression of OPA1 m RNA in paclitaxel group was higher,but the difference was not statistically significant(P>0.05),and the expression of OPA1 m RNA in lung group was significantly increased(P<0.01).Compared with paclitaxel group,the expression of OPA1 m RNA was higher in Feiyanning group,and the difference was statistically significant(P<0.05).(2)Compared with the model group,the expression of MFN2 m RNA in paclitaxel group was significantly increased(P<0.01).The expression of MFN2 m RNA in lung group was higher(P<0.05).Compared with Feiyanning group,the level of MFN2 m RNA was higher in paclitaxel group and the difference was statistically significant(P<0.05).(3)Compared with the model group,the expression level of Drp1 m RNA in the paclitaxel group was lower(P<0.05).The expression level of Drp1 m RNA in the Feiyanning group was significantly lower(P<0.01).Compared with the paclitaxel group,the expression level of Drp1 m RNA in the Feiyanning group was lower than that in the paclitaxel group.Significantly lower,and the difference was statistically significant(P<0.05).(4)The expression levels of Fis1 and MFN1 m RNA were not significantly different between the three groups(P>0.05).5.Protein expression:(1)Compared with the model group,the positive expression level of OPA1 in paclitaxel group was higher,but the difference was not statistically significant(P>0.05),and the positive expression of OPA1 in Feiyanning group was significantly increased(P<0.01).Compared with paclitaxel group,the positive expression level of OPA1 in Feiyanning group was higher,and the difference was statistically significant(P<0.05).(2)Compared with the model group,the positive expression level of MFN2 in paclitaxel group was significantly increased(P<0.01).The positive expression level of MFN2 was higher in Feiyanning group(P<0.05).The positive expression of MFN2 in paclitaxel group compared with Feiyanning group the level was higher and the difference was statistically significant(P < 0.05).(3)Compared with the model group,the expression level of Drp1 in the paclitaxel group and the Feiyanning group was significantly lower(P<0.01).Compared with the paclitaxel group,the positive expression level of Drp1 in the Feiyanning group was lower,and the difference was statistically significant(P<0.05).(4)The positive expression levels of Fis1 and MFN1 protein were not significantly different between the three groups(P>0.05).Conclusions:1.Feiyanning Decoction can effectively inhibit the growth of transplanted tumors in Lewis lung cancer mice and improve the survival of mice.2.After intervention by Feiyanning Decoction,the mitochondria of Lewis lung cancer mice were mostly strip-shaped and strip-like.3.Feiyanning Decoction can down-regulate the expression of Drp1 m RNA in transplanted Lewis lung cancer mice and up-regulate the expression of OPA1 and MFN2 m RNA.4.Feiyanning Decoction can down-regulate the expression of Drp1 protein in Lewis lung cancer xenografts and up-regulate the expression of OPA1 and MFN2 proteins.5.Feiyanning has no regulatory effect on Fis1 and MFN1 signals in Lewis lung cancer xenografts.6.Feiyanning may interfere with the growth of transplanted tumors in Lewis lung cancer mice by interfering with the regulation of Drp1/OPA1 cleavage and fusion signals,interfering with the role of mitochondria in tumors. |
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