Study On The Intervention Of Shen Ge San On Myocardial Hypertrophy Based On TWEAK/Fn14 Signaling Pathway

[Purposes]By using TWEAK/Fn14 signal pathway agonist Ang Ⅱ to establish a stable model of cardiomyocyte hypertrophy,the effects of Shen Ge San on the model of cardiomyocyte hypertrophy were studied.To study the TWEAK/Fn14 signaling pathway and explore the mechanism of Shen Ge San on myocardial hypertrophy and abnormal energy metabolism in rats with pressure overload heart failure.[Methods]1.To observe the toxic effects of Shen Ge San,Ang Ⅱ and valsartan on H9c2 myocardial cells.2.To observe the effect of Ang Ⅱ on H9c2 cardiomyocyte hypertrophy3.To observe the effect of Shen Ge San on the hypertrophy of H9c2 cardiomyocytes induced by Ang Ⅱ.4.To observe the effect of Shen Ge San on Ang Ⅱ-induced hypertrophy of H9c2 cardiomyocyte.Western-blot was used to detect the expression of TWEAK,Fn14 and PGC-1 α in each group.5.To observe the effect of Shen Ge San on the expression of TWEAK,Fn14 and PGC-1α in myocardial tissue of rats with pressure overload heart failure by HE staining and Western-blot.[Result]1.Compared with the blank group,there was no significant difference in the concentration of Shen Ge San 0.003 mg/ml or below(P>0.05).There was no significant difference in the concentration of Ang Ⅱ 1 × 10-6 mol/ml or below(P>0.05).There was no significant difference in valsartan concentration of 1 × 10-6 mol/ml or below(P>0.05).2.Compared with 0 h,Ang Ⅱ induced by 1 × 10-7 mol/ml for 48 h had a significant difference in the relative area of myocardial cells(P<0.05).3.Compared with the blank group,the relative area of H9c2 myocardial cells in Ang Ⅱ group was different(P<0.05).Compared with Ang Ⅱ group,Shen Ge San group and valsartan group could reduce the relative area of myocardial cells with significant difference(P<0.05).4.Compared with the blank group,the expression of TWEAK,Fn14 and PGC-1 a protein in Ang Ⅱ group had statistical significance(P<0.05).Compared with Ang Ⅱgroup,the expressions of TWEAK,Fn14 and PGC-1 α in Shen Ge San group and valsartan group had statistical significance(P<0.05).5.Compared with sham-operated group,cardiomyocyte hypertrophy in model group.Compared with the model group,the hypertrophy of myocardial cells in Shen Ge San group was reduced.6.Compared with sham-operated group,the expression of TWEAK and Fn14 protein in model group had statistical significance(P<0.05).Compared with the model group,the expression of TWEAK and Fn14 protein in Shen Ge San group had statistical significance(P<0.05).Compared with model group,the expression of PGC-1 αprotein in Shen Ge San group had statistical significance(P<0.05).[Conclusion]1.Ang Ⅱ can induce H9c2 cardiomyocyte hypertrophy,which is an important factor in inducing H9c2 cardiomyocyte hypertrophy.2.Shen Ge San can alleviate the hypertrophy of H9c2 myocardial cells induced by Ang Ⅱ.3.Ang Ⅱ induced the expression of TWEAK and Fn14 protein in hypertrophic H9c2 cardiomyocytes to increase,while the expression of PGC-1 α protein was down-regulated4.Shen Ge San can down-regulate the expression of TWEAK and Fn14 in hypertrophic H9c2 cardiomyocytes,and inhibit the decrease of PGC-1 α protein expression.5.Shen Ge San can alleviate the hypertrophy of cardiac myocytes in rats with pressure overload heart failure6.Shen Ge San can decrease the expression of TWEAK and Fn14 in myocardium of rats with pressure overload heart failure,and increase the expression of PGC-1 a.