Effect And Mechanism Of Astragaloside IV On Type 1 Diabetic Female Mice Reproductive System

With the improvement of living standards and the spread of sub-healthy lifestyles,more and more women of childbearing age are suffering from diabetes,which increases the risk of infertility for women of childbearing age.This potential risk of reproductive disorders is closely related to the damage of the ovaries in the environment of high blood sugar.High blood sugar will lead to the production of reactive oxygen species(ROS)in cells.Excessive ROS will destroy the function of intracellular proteins and nucleic acids,which cause tissue damage and tissue aging.As a traditional Chinese medicine,Astragalus is believed to have functions such as invigorate.The in vivo metabolite of astragaloside IV,which is the main component of Astragalus membranaceus,is proven to have the functions of anti-oxidative stress and activating telomerase.Many studies have found that adding astragaloside IV to an in vitro cell culture system can reduce the production of cell ROS,improve cell proliferation,and be effectively used in the treatment of diabetes.But its effect on oocytes has not been reported yet.To further explore the effect of astragaloside IV on oocytes,we use Streptozocin(STZ)to prepare Type 1 diabetes(T1D)model mice,and they were randomly divided into a control group and a treatment group.The treatment group was gavage with a single dose of 50 mg astragaloside IV twice a day for one week,and the control group was gavage with normal saline.Then,the effects of Astragaloside IV on the reproductive system of diabetic female mice were explored from the three dimensions of embryology,histochemistry and molecular biology.The results showed that astragaloside IV gavaging can significantly increase the number of superovulation in T1 D female mice.The average number of ovulations per ovulation in the treatment group was 12.6 ± 1.7,which was significantly higher than that in the model group by 1.6 ± 1.2(P<0.05),and the shape of oocytes is close to normal mice.At the same time,gavage astragaloside IV can also improve the in vitro development ability of oocytes of T1 D female mouse in germinal vesicle(Germinal vesicle,GV)stage.The rate of Germinal vesicle breakdown(GVBD)in the treatment group is 32.9 ± 5.9,the rate of the first polar body(Polar body I,PB1)was 13.±4.1,which is higher than the model group GVBD rate of 13 ± 2.3,the rate of PB1 is 0.8 ± 0.79(P<0.05).ROS detection was performed on the oocytes at each stage of the in vitro development of each group.It was found that the change pattern of ROS in the oocytes of the control group was changed compared with that of the blank group,and the peak appeared in the GV phase.Histochemical experiments verified that the appearance of this ROS peak in GV phase is related to the apoptosis of granular cells,and astragaloside IV can inhibit the apoptosis of granular cells.By constructing a high-glucose granule cell model and molecular biology experiments,we found that gavaging astragaloside IV can inhibit the apoptosis of granule cells in a high-glucose environment through its in vivo metabolite cycloastragaloside.This inhibition is achieved through its telomerase activation effect.