| Objective:Comparison of the general condition between Patients with intestinal tumors and healthy control group.Compare the changes in the number and function of peripheral blood lymphocytes in gastrointestinal cancer patients and control group.Compare the intestinal micribiota changes in Patients with gastrointestinal malignant tumor and healthy control group to determine the correlation between them and the number and function of peripheral blood lymphocytes.Methods:A total of 26 patients with intestinal malignant tumors(including 14cases of gastric cancer,5 cases of colon cancer,7 cases of rectal cancer,male accounting for 53.8%,mesn age 54.81±6.974),And they were all patients of Affiliated hospital of hebei university of engineering from June 2018 to December 2019.30 whose age and gender-matched Healthy Controls(HC)(male accounting for 63.3%,mesn age52.50±6.421).The HC group was recruited from volunteers.Peripheral blood lymphocytes and feces of the above participents were collected.Flow cytometry waas used to detect the number of peripheral blood lymphocytes,and16s technology was used to detect the relative abundance of each flora in feces to analyze the number of lymphocytes and expression of intestinal flora in gastric cancer and HC.we analyze the correlation between the relative abundance of each floa with the number of lymphocytes.Results:1.There was no significant difference in age composition and gender between gastrointestinal cancer patients group and healthy control group.2.There was a significant difference in the number of CD4~+cells in peripheral blood between gastrointestinal malignant tumor group and healthy control group.The number of T cell subsets in group GC(27.49±9.48)was lower than that in group HC(31.94±6.33),P<0.05).3.There was a significant difference in the number of CD8~+cells in peripheral blood between gastrointestinal malignant tumor group and healthy control group.The number of T cell subsets in group GC(30.79±6.71)was significantly higher than that in group HC(26.94±7.15),P<0.05).4.The number of NK cells in peripheral blood of gastrointestinal malignant tumor group and healthy control group(20.95±1.66)was higher than that of healthy control group(7.98±0.26),P<0.05).5.There was a significant difference in the detection of Treg cells in peripheral blood between gastrointestinal malignant tumor group and healthy control group.The number of Treg cells in group GC(1.55±0.34)was significantly higher than that in group HC(0.36±0.24),(P<0.05).6.Compared with the healthy control group,the relative abundance of amoeboid bacteria,actinomycetes and verrucous microbacteria in patients with gastrointestinal malignant tumors increased significantly,while the relative abundance of Bacteroides Pseudomonas decreased significantly in patients with gastrointestinal malignant tumors compared with healthy controls.7.In group GC,The CD4~+cells in peripheral blood were positively correlatedwith intestinal actinomycetes(r=0.419),while in group GC,The NK cells in peripheral blood were positively correlated with intestinal actinomycetes(r=0.50),andsignificantly correlated with Fusobacterium(r=0.658,P<0.05).8.In group GC,The NK cells in peripheral blood were positively correlated with intestinal bacteroides(r=0.493,P=0.001),and significantly correlated with Clostridium(r=0.658,P=0.000).Conclusion:compare with HC,gastrointestinal cancer patients have abnormalities in lymphocyte subsets and bacterial structure,and there is a certain correlation between the changes in the bacterial structure and the changes in lymphocytes,which highlysuggests that the changes in the bacterial structure may be involved in the occurrence and progression of gastrointestinal cancer lymphocyte abnormalities and thedevelopment of diseases.This study Through the early detection of peripheral blood of tumor patients combined with intestinal flora analysis,which provides a certaintheoretical basis for turher clarifying the essect of intestinal flora on lymphocytes and the incidence of gastrointestinal cancer patients. |
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