Expression And Significance Of PLK1 And S100A6 Genes In Nonfunctional Pituitary Adenomas

Objectives:To observe the expression of PLK1 and S100A6 in nonfunctional pituitary adenoma,and explore their correlation with the invasivity of nonfunctional pituitary adenoma,so as to provide help for clinical treatment.Methods:In this study,20 non-functional pituitary adenoma specimens(10 invasive,10 non-invasive)were selected.Using Real-time PCR Detecting policy System to detect PLK1 and S100A6 genes in invasive non-functional pituitary adenomas and noninvasive non-functional pituitary adenomas gene expression differences of two groups of samples between the two;Western blot was used to detect the expression of PLK1 and S100A6 proteins in the two groups.Finally,the results are analyzed and discussed statistically.Results:The results of real-time quantitative PCR showed that S100A6 gene was highly expressed in non-functional pituitary adenoma,and the expression of S100A6 gene in invasive pituitary adenoma was significantly higher than that in non-invasive pituitary adenoma(P < 0.05).However,there was no significant difference in the expression of PLK1 gene between the two groups with invasive and non-invasive non-functional pituitary adenoma(P > 0.05).Western blot results showed that S100A6 protein was highly expressed in the invasive non-functional pituitary adenoma group and moderately expressed in the non-invasive non-functional pituitary adenoma group.PLK1 protein was expressed in both invasive and non-invasive pituitary adenomas,but there was no significant difference.Conclusion: S100A6 gene may be related to the invasiveness of non-functional pituitary adenoma.PLK1 gene may be unrelated to the invasiveness of nonfunctional pituitary adenoma.