The Effect Of WE43 Magnesium Alloy On Cytotoxicity And Osteogenic Effect

Objective: Preliminary study of hemocompatibility of WE43 magnesium alloy and its effect on cytotoxicity and osteogenic differentiation of bone marrow mesenchymal stem cells,with a view to providing WE43 magnesium alloy as a fixture for clinical use to provide relevant experimental reference.Methods: The compatibility experiment is divided into there groups:WE43 magnesium alloy group,TC4 titanium alloy group and blank control group.The hemocompatibility of WE43 magnesium alloy was evaluated by in vitro hemolysis experiment,measurement of in vitro clotting time and observation of platelet adhesion using an electron microscope;BMSCs were cultured from whole bone marrow adherent method,and the morphology and structure of primary and passage;BMSCs were observed and recorded under the microscope;Detection of cell surface markers by flow cytometry;BMSCs were cultured using WE43 magnesium alloy and TC4 titanium alloy extract,CCK-8 method was used to detect the cytotoxicity of WE43 magnesium alloy to BMSCs;Alizarin red staining and q-PCR were used to detect WE43 magnesium Alloying ability to induce osteogenic differentiation of BMSCs.Results:1.The results of hemolysis experiments show that WE43 magnesium alloy and TC4 titanium alloy are not cause hemolytic eactions in vitro.The coagulation time experiment shows that WE43 magnesium alloy and TC4 titanium alloy have good anticoagulant in vitro.WE43 magnesium alloy and TC4 titanium alloy will not activate the coagulation system.Morphology of platelet adhesion observed by scanning electron microscopy shows that both WE43 magnesium alloy and TC4 titanium alloy show the excellent anticoagulant abilities without activating platelets.2.The results of flow cytometry showed that the isolated P3-P4 cells highly expressed non-hematopoietic cell line surface antigens CD29(99.3%),CD90(99.87%),and low expression hematopoietic stem cell surface antigen CD45(1.93%).The reported phenotypes of BMSCs are consistent;3.The results of CCK-8 cell proliferation experiments show that the cells of the WE43 magnesium alloy group,TC4 titanium alloy group and the blank control group have an increasing trend over time.The cell proliferation rate of the WE43 magnesium alloy group is higher than that of the TC4 titanium alloy group and the blank control.The difference was statistically significant(P<0.05);4.After being contacted with WE43 magnesium alloy extract,BMSCs still have the characteristics of stem cells and can still differentiate into osteoblasts.Alizarin red staining and PCR detection of osteogenesis-related factors: Col1,OCN content test verified that BMSCs osteogenic differentiation was higher than TC4 titanium alloy group and negative control group,and the difference was statistically significant.Conclusion: WE43 magnesium alloy has good Hemocompatibility,and has no obvious cytotoxicity to bone marrow mesenchymal stem cells,maintains its osteogenic differentiation potential and can promote its osteogenic differentiation ability.