Effects Of Genital Tract Ureaplasma Urealyticum Positive On Pregnancy Outcomes After IVF/ICSI-ET

Objective: To investigate the effects of genital tract Ureaplasma Urealyticum positive on the pregnancy outcome of in vitro fertilization/ intracytoplasmic sperm injection-embroy transfer cycles.Methods: Retrospective analysis was carried out on 559 infertile patients receiving IVF/ICSI-ET treatment for the first time at the Reproductive Center of The Second Affiliated Hospital of Chongqing Medical University from January 2017 to January 2019(278 fresh embryo transfer cycles,281 frozen-thawed embryo transfer cycles).The patients were divided into four groups(female and male both positive group?only female positive group?only male positive group?female and male both negative group)according to the results of culture of UU in genital secretions.Baseline characteristics ? laboratory data and pregnancy outcomes were compared among the four groups.Multifactor Logistic regression was used to analyze the risk factors that affect the pregnancy rate.Results: Among 559 infertile couples,female UU positive rate was 52.77%,male positive rate was 31.13%.There were no significant differences in the rate of previous ectopic pregnancy and the tubal factor infertility in fresh embryo transfer cycles(15.9% vs 20.8% vs 18.8% vs 10.2%;57.1% vs 55.6%vs 50.0% vs 42.5%,P>0.05),These differences were also not observed in frozen-thawed embryo transfer cycles(22.1% vs 21.7% vs 16.7% vs 20.4%;31.2% vs 36.1% vs 33.3% vs 35.9%,P>0.05),there were no significant differences in other baseline characteristics between the four groups(P > 0.05).There were no significant differences in normal fertilization rate?cleavage rate?quality embryo rate and blastocyst formation rate among the four groups(P > 0.05).In the fresh embryo transfer cycles,both positive group had mildly lower clinical pregnancy rate and live birth rate when compared with other three groups,,but there were no significant differences(50.8% vs 52.8% vs 56.3% vs 58.3%;36.5% vs 47.2% vs 43.8% vs 49.6%,P > 0.05),there were no significant differences in clinical pregnancy rate and live birth rate in frozen-thawed embryo transfer cycles(46.8% vs 45.8% vs 50.0% vs 47.6%;29.9% vs 33.7% vs 33.3% vs 29.1%,P > 0.05).The multiple pregnancy rate?abortion rate?ectopic pregnancy rate?premature rupture of membranes rate?preterm delivery rate?small for gestation age rate were compared among the four groups in fresh embryo transfer or frozen-thawed embryo transfer cycles had no significant differences(P > 0.05).Multivariate Logistic regression analysis showed that women's age(OR=0.923,95%CI=0.888~0.960,P < 0.001)and number of implanted embryos(OR=2.094,95%CI=1.077~4.073,P=0.029)were influence factors for IVF/ ICSI-ET pregnancy rate.Conclusion: Ureaplasma Urealyticum positive in the genital tract of both men or women had no adverse effect on pregnancy outcomes in IVF/ICSI-ET.Multivariate Logistic regression analysis showed that the pregnancy rate was negatively correlated with the female age and positively correlated with the number of embryos implanted.