Effects Of Curcumin Nanoparticles Combined With Focused Ultrasound On Glioma Cells

PART Ⅰ STUDY ON THE PREPARATION OF URCUMIN-PLGA NANOPARTICLES AND THEIR FFECT ON APOPTOSIS IN GLIOMA CELLSAim To prepare curcumin-PLGA nanoparticles(curcumin poly(lactic-co-glycolic acid)nanoparticles,Cur-PLGA-NPs)and evaluate their effects on glioma cells in vitro.Methods C6 cells and U251 cells were subcultured and randomly divided into control group,curcumin group and curcumin-PLGA group.The morphology of Cur-PLGA-NPs was observed by inverted fluorescence microscope and transmission electron microscopy.The particle size of Cur-PLGA-NPs was detected by Malvern particle size potentiometer.The encapsulation efficiency and drug loading rate of Cur-PLGA-NPs were detected by a multi-function microplate reader.Fluorescence microscopy was used to observe the uptake of Cur-PLGA-NPs by C6 cells and U251 cells.CCK-8 was used to detect the effect of Cur-PLGA-NPs on the viability of C6 cells and U251 cells.Flow cytometry was used to detect the effect of Cur-PLGA-NPs on apoptosis.Apoptosis was detected by Hoechst staining.Results The Cur-PLGA-NPs have an average particle size of(284.6± 9.0)nm and are spherical.The encapsulation efficiency was(70.712 13± 2.614 663)%,and the drug loading rate was(2.828 485±0.104 587)%.Compared with the control group and the curcumin group,C6 cells and U251 cells significantly increased the uptake of Cur-PLGA-NPs(P<0.05).Compared with the control group and the curcumin group,the cell viabilities of C6 cells and U251 cells in the curcumin-PLGA group were significantly decreased(P< 0.05).Compared with the control group and the curcumin group,the Cur-PLGA-NPs induced apoptosis significantly in C6 cells and U251 cells(P< 0.05).Conclusion Compared with curcumin,Cur-PLGA-NPs can enhance the uptake of drugs by tumor cells,promote tumor cell apoptosis.PART Ⅱ EFFECTS OF CURCUMIN NANOPARTICLES COMBINED WITH FOCUSED ULTRASOUND ON GLIOMA CELLSObjective To evaluate the effect of curcumin nanoparticles combined with focused ultrasound on glioma cellsMethods Randomly divided into control group,curcumin nanoparticles group,focused ultrasound group and curcumin nanoparticles combined focused ultrasound group.CCK-8 was used to detect the effect of each experimental group on the activity of U251 cells;live and dead cell staining was used to detect the survival of cells in each experimental group;SOSG stain was used to detect the production of singlet oxygen;DCFH-DA staining was used to detect the generation of intracellular reactive oxygen species in each experimental group.Results Compared with the control group,curcumin nanoparticle group,and ultrasound group,the CCK8 and live-dead cell staining results showed that the nanoparticle combined with focused ultrasound group could cause cell death more significantly(P <0.05).The results of SOSG staining showed that the singlet oxygen produced by curcumin nanoparticles combined with focused ultrasound group was significantly igher than that of blank control group,and increased with the increase of curcumin nanoparticles concentration,ultrasonic power and ultrasonic irradiation time(P <0.05).DCFH-DA staining showed that curcumin nanoparticles combined with focused ultrasound group can produce a large amount of intracellular reactive oxygen species compared with other groups.Conclusion Compared with the control group,curcumin nanoparticle group,and focused ultrasound group,the curcumin nanoparticle combined with focused ultrasound group can enhance the killing effect on glioma cells.The mechanism may be related to the large amount of intracellular reactive oxygen species.