Effect Of Teng Liquqi-Fang On Proliferation And Apoptosis In Ovarian Cancer SKOV-3 Cells

Objective To investigate the effect of Teng Liquqi-Fang(TLQQF)on the proliferation and apoptosis in ovarian cancer SKOV-3 cells.Method 48 SPF male rats weighing 220 ± 20 grams were randomly divided into four group,including a normal saline group(normal saline gavage),cisplatin group(intraperitoneal injection of cisplatin),TLQQF group(TLQQF gavage)and TLQQF combined with cisplatin group(TLQQF gavage combined with intraperitoneal injection of cisplatin).Blood was taken from the heart after continuous gavage for 5 days in rats.SKOV-3 cells were purchased and divided into a blank group and an experimental group.The experimental component group was divided into drug-containing serum groups.After 24 hours and 48 hours of intervention,they were used for detection.MTT assay was used to detect the effect of different concentrations of TLQQF on the inhibitory growth of ovarian cancer SKOV-3 cells.Hematoxylin-eosin staining was used to observe the changes of cell morphology.Cell cycle and apoptosis were detected by flow cytometry.The expression of level Cyt c,Caspase-9,Caspase-3 and PDCD4 was detected by Western Blot.Results1.Effects of different concentrations of TLQQF and the TLQQF combined with the cisplatin on the inhibitory growth of ovarian cancer SKOV-3 cells:Compared with the normal saline group,the inhibition ratio of the cisplatin group,the TLQQF group,the TLQQF combined with the cisplatin group all increased,and the difference was statistically significant(P <0.01).Compared with the cisplatin group,the TLQQF group,the inhibition ratio of the TLQQF combined with the cisplatin group increased,the difference was statistically significant(P<0.05).2.Effect of TLQQF and TLQQF combined with the cisplatin on morphological changes of ovarian cancer SKOV-3 cells: Compared with the normal saline group,the morphology of the cells changed after the intervention with the drug-containing serum,the nucleolus was clear,the nucleus was pyknosis,and the morphological characteristics of apoptosis were observed.3.Effect of TLQQF and TLQQF combined with the cisplatin on cell cycle and apoptosis of ovarian cancer SKOV-3 cells: The results of cell cycle showed that the ratio of cells in G0 / G1 phase increased after intervention with drug-containing serum in each group,the S phase ratio decreased,and the difference was statistically significant(P <0.05).Apoptosis results showed that compared with the normal saline group,the apoptosis rate of the cisplatin group,the TLQQF group,the TLQQF combined with the cisplatin group was significantly increased,and the difference was statistically significant(P <0.05).Compared with the cisplatin group and the TLQQF group,the apoptosis rate of the TLQQF combined with the cisplatin group increased,the difference was statistically significant(P <0.05).4.Changes of Cyt C,Caspase-9,Caspase-3 and PDCD4 protein expression in TLQQF and TLQQF combined with the cisplatin on SKOV-3 cells: The proteinaceous expression level of cisplatin group,the TLQQF group,the TLQQF combined with the cisplatin group were higher than that of normal saline group,the difference was statistically significant(P < 0.01).The proteinaceous expression level of TLQQF combined with the cisplatin group was higher than thatof cisplatin group and TLQQF group,the difference was statistically significant(P<0.05).Conclusion1.The TLQQF group and TLQQF combined with the cisplatin group can inhibit the proliferation of human ovarian cancer SKOV-3 cells and promote the apoptosis of SKOV-3 cells.2.The TLQQF and TLQQF combined with the cisplatin can induce apoptosis of ovarian cancer SKOV-3 cell.The possible mechanism is to activate mitochondrial pathway and regulate the expression of Cyt c,Caspase-9,Caspase-3and PDCD4.