| Background Parkinson’s disease(Parkinson’s disease,PD)is an irreversible,progressive neurodegenerative disease,there is not a cure,so the early intervention and treatment can effectively delay the progression,and early diagnosis is very important.In the past 10 years,there are many kinds of potential biomarkers have been proposed,and some of them the feasibility is very high,the alpha-synuclein(alpha-syn)and DJ-1 protein are research hotspots,but whether they could be biological markers for early screening of PD doesn’t have a unified conclusion.Purpose 1.Determined the expression of α-syn and DJ-1 protein in saliva,as a way used for auxiliary diagnosis of Parkinson’s disease(PD).And to explore whether α-syn and DJ-1 protein in saliva have the potential of being a biomarker for early screening of PD.2.Application of statistical software analysis Statistical differences in the concentration of Salivary α-syn and DJ-1 protein between Parkinson’s patients and normal controls.The research analysis the correlation between the concentration of Salivary α-syn and DJ-1 protein and age,gender,the severity of condition on Parkinson’s disease.Methods Through the case-control study,we collected saliva samples from 27 patients with Parkinson’s disease and 27 healthy volunteers,determined and detected the expression of salivary α-syn and DJ-1 protein,and analysed the correlation between the concentration of Salivary α-syn and DJ-1 protein and age,gender,Hohn-Yahr stage,unified Parkinson’s Disease Rating Scale(UPDRS)-II / III score,olfactory score,Montreal cognitive assessment(Mo CA)score,Mini Mental State Examination(MMSE)score in two groups.Results 1.Mean concentration of salivary α-syn in patients(1269.02±16.09)(pg/ml)were significantly lower than in controls(1350.51±25.79)(pg/ml),and mean concentration of salivary DJ-1 protein in Parkinson disease group(6.07 + 3.23)(ng/ml)was significantly lower than the control group(8.43±4.33)(ng/ml),differences were statistically significant(P<0.05).2.Receiver operating characteristic curve(ROC curve)revealed 59.3% sensitivity and 81.5% specificity in separating PD and healthy controls with a cut-off walue of the concentration of α-syn =1325.86(pg/ml).As well as the concentration of DJ-1 protein =7.35(ng/ml)was used for a cut-off point to separating PD and healthy controls,the sensitivity was 77.8%,specificity was 55.6%.3.Correlation analysis showed that,in Parkinson’s disease group,age,gender,UPDRS-II / III score,Hohn-Yahr stage,olfactory score,MMSE score and Mo CA score of Parkinson’s disease group were not related to the concentration of α-syn and DJ-1 protein in saliva(P>0.05).Salivary α-syn and DJ-1 protein concentrations in male patients were both lower than in women patients,but the difference wre not statistically significant(t=0.609,P=0.548;t=0.333,P=0.742).4.In the control group,there were no significant difference in age,gender and α-syn and DJ-1 protein concentration in saliva(P>0.05).Conclusions 1.In Parkinson’s disease group and control group the salivary α-syn and DJ-1 protein could be detected,and mean concentration of salivary α-syn and DJ-1 protein in patients were lower than in controls,the difference was statistically significant.2.Detected the concentration of salivary α-syn and DJ-1 protein were suitable for assessing condition of China Parkinson’s patients,to distinguish between Parkinson’s disease and normal volunteers the sensitivity and specificity were 59.3%、81.5%(α-syn =1325.86pg/ml),77.8%,55.6%(DJ-1 protein =7.35ng/ml).3.The concentration of α-syn in saliva of patients with Parkinson’s disease may be related to Hohn-Yahr stage,which was positively correlated.4.Quantitative detection of the content of α-syn and DJ-1 protein in saliva could be a convenient,accessible and non-invasive method for early screening patients with Parkinson’s disease. |
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