Screening Of Traditional Chinese Medicine And Mechanism Against Triple Negative Breast Cancer Based On Second Generation Sequencing

Objective: A worldwide problem in the treatment of breast cancer is the lack of specific drugs in the clinical treatment for triple negative breast cancer.In this study,MDA-MB-231 cells were used as the animal model,and high-throughput screening technology based on second-generation sequencing was applied to find the anti-triple negative breast cancer effect of traditional Chinese medicine residual ganesia,and the mechanism of residual negative breast cancer was further studied.Methods:1.High-throughput screening technology based on second-generation sequencing was used to find traditional Chinese medicine residues,which have activity for anti-triple negative breast cancer.The genes were predicted up-regulated: Cyclin G2,P21.down-regulated: Cyclin B,ATG8,and atogin-1 by KEGG pathway analysis.2.The experimental group and the control group were set up and the human triple negative breast cancer line MDA-MB-231 cells were cultured in vitro.The cell aviability of MDA-MB-231 cells was detected by tetrazolium blue(MTT)collimation after 24 h treatment with residual gan(320、160、80、40、20、10、5 μg/m L)and IC50 was calculated.3.Flow cytometry was used to analyze the cell cycle changes of MDA-MB-231 cells after 24 h of treatment with residual gunine(20 μg/m L).4.The migration ability of MDA-MB-231 cells was determined by cell scratch experiment after treatment of residual gunine(20 μg/m L).5.Expression of Cyclin G2 and cyclin-dependent protein kinase inhibitor p21 in MDA-MB-231 cells treated by residual gan(20 μg/m L)for 24 h were detected by real-time PCR.6.KMplot database was used to analyze the expression and prognosis of Cyclin G2 and p21 in triple negative breast cancer patients.Results: Compared with the control group,the treatment group had a significantly inhibitory effect in MDA-MB-231 cells in vitro(P <0.05),and the inhibitory effect was concentration-dependent.The proliferation of human breast cancer MDA-MB-231 cells was inhibited in vitro and cell cycle was blocked in G1/S.The expression levels of Cyclin G2 and p21 m RNA in MDA-MB-231 cells were significantly upregulated by residual gan(20μg/m L)(P <0.05).KMplot analysis of the clinical data suggested that high expression of Cyclin G2 and p21 could potentially increase the prognosis of triple negative breast cancer patients.Conclusion: The high-throughput screening technology based on second-generation sequencing was used to detect the anti-triple negative effect of Chinese traditional medicine(20 μg/m L)on breast cancer,and the mechanism may be related to the up-regulation of Cyclin G2 and p21 m RNA levels.