Study On The Mechanism Of LncRNA NEAT1 In PHI Effect On Apoptosis In M2b Leukemia Cells

Objective: To investigate the effect of PHI on kasumi-1 cell line,an acute myeloid leukemia(AML M2b)in vitro.The key Lnc RNA targets involved in the PHI against AML M2 b was determined.The relationship between P53 pathway and the key Lnc RNA in the PHI effect on kasumi-1 cells was studied.Methods:(1)Inhibition cell growth by PHI was determined by CCK8.Cell apoptosis measured by Flow cytometry.The expression of Lnc RNA NEAT1 was checked by q PCR.(2)The different si RNA segments targeting NEAT1 were designed and transfected into kasumi-1 cells by lipofection.The best NEAT1-si RNA fragment was selected by transfection efficiency.Proliferation and apoptosis of kasumi-1 cells after exposure to PHI and NEAT1-si RNA transfection was detected by CCK8 method and Flow cytometry.(3)Lnc RNA-NEAT1 after co-incubation of PHI and P53 inhibitor(Pifithrin-α)was checked by q PCR.The expression of P53 protein after co-treated with PHI and P53 inhibitors,which transfected with Lnc RNA-NEAT1 or non transfectde were detected by Western blot.Result: PHI could inhibit proliferation of Kasumi-1 cells and induce apoptosis in a concentration dependent manner.The Lnc RNA-NEAT1 improved by PHI.Silencing NEAT1 reverses PHI effect on proliferation and apoptosis in Kasumi-1 cells.P53 inhibitor(Pifthrin-α)inhibited NEAT1,which was upregulated PHI.PHI up-regulate P53 proteins in Kasumi-1 cells,which prevented by P53 inhibitor and silencing NEAT1.Conclusions: NEAT is the key Lnc RNA in the effect of PHI on the proliferation and apoptosis of Kasumi-1 cell line.Silencing NEAT1 reverses PHI effect on proliferation and apoptosis in kasumi-1 cells.The Lnc RNA-NEAT1 is inhibited by P53 inhibitor,indicating that it might be a downstream target of P53.The mechanism of PHI effect on AML M2 b might upregulae NEAT1 via the activation of the P53 pathway.