Effects Of The Aerobic Exercise On The Pulmonary Inflammation Response And Chemerin/CMKLR1 In The Rats With COPD

Research ObjectiveChronic obstructive pulmonary disease(COPD)is characterized by the chronic,progressive and gradually aggravating cough,sputum and dyspnea,which severely affects the lung function and life quality of the patients.Chronic inflammation is the most important pathogenesis of impaired lung function in the patients with COPD.chemerin / CMKLR1 has been reported to be involved in chronic inflammatory response in COPD.The aerobic exercise is the most commonly used rehabilitation method in the pulmonary rehabilitation management of COPD,and it has been reported that the aerobic exercise could improve the chronic pulmonary inflammation of COPD,but the specific mechanism is still unclear.Therefore,this study exerted exercise intervention on the model rats with COPD to explore the effect of the aerobic exercise on the pulmonary inflammatory response and chemerin / CMKLR1 of the rats with COPD,thus further revealed the mechanism of using exercise to prevent and treat COPD.Research MethodThe SD rats were established as COPD models by exposure to cigarette smoke.After successful modeling,the rats with COPD were randomly divided into control group and aerobic exercise group,in which there were 7 rats.The aerobic exercise group received treadmill exercise intervention lasting 9 weeks: the exercise speed was 13.3 m / min.The adaptive training was given in the first week,there was 10 minutes in a day and 3 days in a week.The formal training was given in the second to ninth weeks,there was 40 minutes in a day and 3 days in a week.The control group did not have any intervention.After the exercise intervention,the rats were anesthetized intraperitoneally.The lung function of rats was measured by Buxco lung function test system,and the lung tissues were taken for morphological and molecular biological examination.The HE staining was used to observe the pathological changes of lung tissue,and the western blot was used to detect the change of expression level of chemerin,CMKLR1,IL-1β,IL-6,IL-8 and TNF-α protein in the lung tissue of rats.Research Results(1)In the control group,there were a lot of inflammatory cells infiltrating around the bronchus,the secretion of mucus in the airway increased,the hyperplastic mucosa protruded into the lumen,the wall of the tube thickened,and the alveolar structure was destroyed obviously,and the alveolar septa were ruptured and fused to form the pulmonary bullae,which was consistent with the pathological manifestations of chronic bronchitis and emphysema.In the aerobic exercise group,the inflammation infiltration around the bronchus reduced,the secretion of mucus in the airway reduced,the hyperplasia of the mucosa was reduced,the wall of the bronchus became thinner,the destruction of the alveolar structure reduced,and the cross-sectional area of the alveoli decreased significantly.These results suggested that the symptoms of chronic bronchitis and emphysema decreased.(2)After two months of treadmill exercise,compared with the control group,the test of lung function showed that FEV100,FEV200,PEF and MMEF significantly increased in the the rats in the aerobic exercise group(P< 0.05),FVC and IC had an trend to increase,but the difference was not statistically significant.(3)After two months of treadmill exercise,compared with the control group,the expression of chemerin protein in the lung tissue of the the rats in the aerobic exercise group significantly increased(P< 0.05),but the expression of CMKLR1 protein significantly decreased(P< 0.05).(4)After two months of treadmill exercise,compared with the control group,the expression of IL-8 protein in the lung tissue of the the rats in the aerobic exercise group significantly decreased,and the difference was statistically significant(P< 0.05).The expression of IL-1β,IL-6 and TNF-α protein decreased,but there was no statistical difference.Research ConclusionAerobic exercise significantly improved the lung function of rats with COPD,and could reduced the pathological damage of the lung tissue in the rats with COPD.After aerobic exercise,the expression of chemerin increased,the expression of receptor CMKLR1 and inflammatory factors decreased in the lung tissue of the rats with COPD,which suggested that exercise has anti-inflammatory effect.We hypothesized that aerobic exercise could improves lung injury in rats with COPD,which was in association with the chronic inflammatory signaling pathways chemerin / CMKLR1 involved.