| According to the statistics of the World Heart Federation,cardiovascular disease has become one of the major diseases threatening human life and health in the world today.It is the biggest obstacle of the second health revolution.Cardiovascular diseases are the circulatory system(heart,microvasculature,arteries and veins)problems,including arrhythmia,congenital heart rate irregularity,heart failure,coronary heart disease,and etc.Heart rate and blood pressure are important physiological parameters of human,which associated with cardiovascular function.At the present stage,there is no good way to cure cardiovascular disease.Treatment is largely dependent on both general drug administration and prevention.Therefore,in order to find a cure for cardiovascular diseases and study the pathogenesis,the cardiovascular functional parameters of model organisms should first be investigated during their development.Locusta migratoria has its advantages to be a model organism and is a powerful model for studying cardiovascular development and gene function.Optical coherence tomography(OCT)is a relatively new imaging technology with the characteristics of non-invasive,real-time and high-resolution.It can obtained the high resolution 3D image of internal structure of sample by backscattering light acquisition,which is based on the interference characteristics of weakly coherent light.OCT technology can be applied to both morphological and functional imaging of the organism development.However,there were less relevant literatures investigate the model organism of insect,especially for monitoring of cardiac functional characteristics throughout its life cycle.Therefore,in this paper,OCT technology was used to monitor the functional characteristics of heart during the whole development process of insects,which is helpful to the research of cardiovascular disease.In this study,OCT technology was used for the first time to observe the cardiac development of locusta migratoria during its whole life cycle,and recorded the changes of physiological parameters at different developmental stages.As one of the basic characteristics of the living phenomenon,biological rhythm is associated with the physiological functions of the cardiovascular system.Therefore,the circadian rhythm of adult migratory locusts were monitored in this study.The results show that the heart rates were higher in the day than that in the night.Finally,the biological rhythm are affected by the temperature and light.Therefore,the physiological parameters of locust heart were measured in different temperature and light cycle.Clear and high-resolution microangiography is extremely important for the prevention and detection of early cardiovascular disease.In this study,an optimization speckle variance algorithm was proposed to classify the dynamic blood flow and static tissue before data processing.In order to verify the feasibility of this algorithm,angiographic imaging was performed on the vascular region of nude mice,and the image quality of angiography was analyzed quantitatively. |
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