Separation,Purification,Study On Biological Activity Of Polygonatum Polysaccharide And Preparation Of Granules

Polygonatum sibiricum is the dried rhizome of Liliaceae,which is a traditional Chinese medicine used for both medicine and food in China.Polygonatum contains polysaccharides,steroidal saponins,flavonoids and other components,which can enhance immunity,antioxidant,anti-tumor,regulation of blood glucose and blood lipids and other pharmacological effects.Polygonatum contains polysaccharides,steroidal saponins,flavonoids and other components,which can enhance immunity,anti-aging,antibacterial,antioxidant,anti-tumor,regulation of blood glucose and blood lipids and other pharmacological effects.In this paper,the extraction,purification,structure,biological activity and granule preparation technology of Polygonatum polysaccharide were studied.The specific results are as follows:(1)The technological parameters of enzymatic extraction of Polygonatum polygonatum polysaccharides were optimized by response surface method,and the optimum process parameters were obtained as follows: cellulase addition 2.1%,time 51 min,temperature 54℃,pH 5.0,and the extraction rate of Polygonatum polygonatum polysaccharides was 29.54%.(2)The protein removal effect of acid protease was the best among the selected protein removal methods.Under the conditions of pH 3,enzymatic hydrolysis temperature 50 ℃,enzymatic hydrolysis time 40 min and enzyme addition 4%,the protein removal rate was 89.12% and the polysaccharide retention rate was 94.86%.The decolorization effect of macroporous resin D101 is the best among the selected decolorization methods.Under the conditions of temperature 50℃,time 2 h,resin addition 5 g/ 100 mL,the decolorization rate is 89.87%,and the polysaccharide retention rate is 81.64%.Using distilled water and 0.5 mol/L NaCl solution as eluent,PSP-1 and PSP-2 were obtained by DEAE-52 cellulose column chromatography.PSP-1S and PSP-2S were further purified by SephadexG-100 dextran gel column.(3)Infrared spectrum results show that both PSP-1S and PSP-2S have characteristic peaks of polysaccharides and have α-type glycosidic bonds;the relative molecular weights of PSP-1S and PSP-2S determined by gel chromatography are 3.31 KDa and 3.73 KDa,respectively.The results of ion chromatography showed that PSP-1S was composed of arabinose,galactose,glucose,xylose,mannose,fructose,galacturonic acid and glucuronic acid,while PSP-2S was composed of arabinose,rhamnose,galactose,glucose,xylose,mannose,fructose,galacturonic acid and glucuronic acid,and the two components are mainly composed of glucose and galactose,in which PSP-1S contains 75.535% glucose and 12.844% galactose;PSP-2S contains 62.424% glucose and 15.842% galactose.(4)In vitro hypoglycemic study showed that PSP-1S and PSP-2S had certain hypoglycemic effect.When the mass concentration was 3 mg/mL,the inhibition rate of PSP-1S on α-amylase activity was 49.20% and the IC50 was 3.04 mg/mL;the inhibition rate of PSP-1S on α-Glucosidase activity was 55.90% and the IC50 was 2.27 mg/mL;the inhibition rate of PSP-2S on α-amylase activity was 31.17% and the IC50 was 5.25 mg/mL,the inhibition rate of PSP-2S on α-glucosidase activity was 40.17% and the IC50 was 4.13mg/mL.In vitro antioxidant study showed that PSP-1S and PSP-2S had certain antioxidant effect.When the mass concentration was 5 mg/mL,the scavenging rate of PSP-1S on DPPH free radicals was 47.31%,the IC50 was 6.51 mg/ml,and the antioxidant equivalent was 4.20 umol TE/1 mg PSP,the scavenging rate of PSP-1S on ABTS free radicals was 50.48%,the IC50 was 5.14 mg/mL,and the antioxidant equivalent was 4.41 umol TE/1 mg PSP;the scavenging rate of PSP-2S on DPPH free radicals was 38.30%,the IC50 was 11.84 mg/mL,and the antioxidant equivalent was 3.40 umol TE/1 mg PSP;the scavenging rate of PSP-2S on ABTS free radical was 43.49%,the IC50 was 7.65 mg/mL and the antioxidant equivalent was 3.80 umol TE/1 mg PSP.(5)The optimum granulation conditions are as follows: the ratio of raw materials and auxiliary materials is 1: 2,the mixture of soluble starch and mannitol is 1: 1,85% ethanol as wetting agent.The prepared particles can be completely dissolved in hot water in 5 min,,the total amount of Pass through No.1 sieve and can’t pass No.5 sieve was 6.52% < 15%.,and the moisture content is 5.31% < 8%,which meets the requirements of the 2015 edition of Chinese Pharmacopoeia.