Epidemiological Analysis And Biofilm Research Of Acinetobacter Baumannii

【Objective】 To understand the epidemiological characteristics of Acinetobacter baumannii and its ability of biofilm formation,which provides a reference for clinical prevention and treatment of Acinetobacter baumannii infection and control of its biofilm formation.【Methods】 101 strains of Acinetobacter baumannii from patient specimens and related clinical data were collected and analyzed for drug resistance;the strain was genotyped by REP-PCR;the ability of the strain to form biofilm was detected by crystal violet staining;the carrying status of biofilm formation-related genes(bap,abaI,bfmS,csuAB,csuA,csuC,csuD,csuE,cpaA,and ompA)was detected by PCR.【Results】 Acinetobacter baumannii has the lowest resistance rate to Minocycline(11%);the first treatment with Cefoperazone/Sulbactam + Minocycline,or Tigecycline + Carbapenem was superior to other regimens,with statistical differences(P< 0.05);82% of the strains were of the same ERIC type;the positive rate of biofilm formation at 25℃ was significantly higher than that at 35℃;the relationship between the resistance rate to Ampicillin/Sulbactam or Aminoglycosides and the biofilm formation ability shows statistical differences(P<0.05).【 Conclusion 】 Most Acinetobacter baumannii isolates are resistant to multiple antibiotics;Cefoperazone/Sulbactam + Minocycline,or Tigecycline + Carbapenemcan be used as the first choice for the treatment of Acinetobacter baumannii in our hospital;101 strains can be divided into 9 genotypes,type A is the main epidemic type;the resistance rate of strains with strong biofilm formation ability to Ampicillin/sulbactam and Aminoglycosides was significantly lower than that of strains with positive or negative biofilm formation ability.