| Background:Recent years,because of the worsening air quality in many countries,the governmental departments have taken corresponding measures,but the air quality has not improved significantly.Many epidemiological studies have shown that long-term or short-term exposure to atmospheric particulate matter is positive related to the cardiovascular prevalence.The classic injury mechanism is that particulate matter causes elevated ROS to induce cardiovascular disease.Objective:From the prospective of nutrition,the population experiment explored whether Vitamin C has the ability to protect the cardiovascular system through exposure to atmospheric particulate matter.Methods:1.Participants recruitmentThis study adopted a randomized double-blind controlled experiment design.58 healthy adult subjects were recruited from Hebei Medical University,and informed consent was signed.All participants were random Ly divided into a Vitamin C intervention group and a placebo control group.2.Experimental designAll participants were measured for height,weight,waist circumference,hip circumference,blood pressure,calculated body mass index(BMI),collected 5m L of blood from peripheral veins and detected serum biomarkers.In the formal experimental stage,the intervention group performed the experiment in the order of vitamin C→ washout period→ placebo,and the control group performed the experiment in the order of placebo→ washout period→ vitamin C.Each stage of the experiment(same group was taken orally Vitamin C or oral placebo)is 1 week and the elution period is 2 weeks.Vitamin C or oral placebo)is 1 week and the elution period is 2 weeks.3.Environment monitoringDuring the experiment,environmental detectors were used to record the daily PM2.5 and PM10 concentrations.At the same time,the PM2.5,PM10 concentrations,and the temperature and relative humidity were recorded daily at the Hebei Environmental Protection Agency.4.Dietary recordEach experimental period each participant recorded 3 days 24 hours of dietary records(2 working days and 1 weekend)to calculate dietary vitamin C intake.5.Statistical methodsSPSS 21.0 was used for statistical analysis,and the measurement data was expressed as ?X ± S.In order to compare the difference in health outcomes between the intervention group and the control group,a paired t-test was used for statistical analysis.In order to compare the differences in health outcomes measured repeatedly between the two experimental phases,a linear mixed-effects model was used to statistically analyze the potential protective effects of vitamin C.In the health outcome indicators,blood pressure was expressed as the absolute change after intervention and 95% CI.Blood biomarkers were expressed as the relative change percentage and 95% CI after the intervention.The inspection level took both sides,α = 0.05.Result:1.participants baseline results During the experiment,two subjects in the vitamin C intervention group dropped out.Finally,28 subjects in the vitamin C intervention group and 30 subjects in the placebo control group completed the experiment.There were no significant differences in blood pressure and blood biomarkers between the two groups(P>0.05).2.Evaluation of vitamin C protective effects The results of the linear mixed effects model showed that in terms of blood pressure,systolic blood pressures and pulse pressure decreased after intervention,and the difference was statistically significant(P=0.021,0.029,respective).In terms of oxidative stress indicators,Glutathione peroxidase(GSH-PX)activity increased after intervention.The difference was statistically significant(P=0.002).In terms of inflammatory factors,interleukin-6,tumor necrosis factor α,and C-reactive protein decreased after intervention,and the difference was statistically significant(P<0.001,respective).Conclusions:Vitamin C may reduce cardiovascular damage caused by exposure to environmental pollution.The mechanism may be vitamin C reduces the body’s ROS level,protects the activity of antioxidant enzymes,and reduces the level of inflammatory factors. |
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