The Role Of OPG/RANK/RANKL And MiR-217 In The Proliferation And Fibrosis Of Giant Cell Tumors Of Bone

Objective: Giant-cell tumor of bone(GCTB)is one of the common types of primary tumor.Nevertheless,the pathogenesis of GCTB is still indistinct,and the high recurrence rate of the GCTB makes it a tough problem.For now,several research results have proved that GCTB is positively correlated with Micro RNA,but the exact regulatory mechanism remains unclear.The purpose of this research is to discuss the possible correlation between Micro RNA and proliferation as well as fibrosis of GCTB.This paper will discuss the role and mechanism of Micro RNA in the occurrence and development of GCTB by means of studying OPG/RANK/RANKL signal paths as well as the proliferation and fibrosis of GCTB.Method: The research adopted the method of in vitro culture of GCTB cells,cultured cells were divided into two groups.Group A was the control group and group B.The protein and m RNA expressionsof OPG,RANK and RANKL were tested separately after the cell culture.Statistical analysis of the correlation between microrna-217 and the above indicators.Quantitative PCR and Western blot were used to detect the expression changes of mir-217,OPG,RANK and RANKL in giant cell tumor cells.Cell proliferation was detected by MTT and brdu staining.Transwell was used to detect invasion.Expressions of e-cadherin,n-cadherin and vimentin were detected by Western blot.The relationship between mir-217 expression changes and proliferation and fibrosis of giant cell tumor was analyzed by the above techniques.Finally,statistical methods were applied to analyze whether there was a statistical difference between the experimental group and the control group at each time point.Results:(1).MiR-217 has high level of gene expression in osteoblast hFOB 1.19.OPG has high level of gene expression in GCTB cells GCT0404(2).OPG has high level of gene expression in GCTB cells;RANK and RANKL have high levels of gene expression in h FOB cells(3).When mir-217 is highly expressed,the number of GCTB cells will decrease,cell survival rate will decrease,and cell proliferation will be inhibited(4).Mir-217 was highly expressed,which could inhibit the invasion of GCTB cells(5).In GCTB cells,mir-217 increased e-cadherin expression,while decreased n-cadherin and Vimentin expression.Conclusions:(1).MicroRNA is related to the occurrence and development of GCTB.(2).During the occurrence stage,the protein and m RNA expression levels of RANK and RANKL are low.(3).During the occurrence stage,miRNA-217 inhibits the proliferation and invasion of GCTB by means of manipulating OPG/RANK/RANKL signal paths and related factors,proteins and m RNA,thus might prevent the relapse of tumor.