| Objects Based on Cys LTs inflammatory pathways to explore the mechanism of the effect of Dioscorea nipponica Makino on bronchial asthma.Methods Fifty SD rats were used,all of which were female.They were divided into 5groups according to the random grouping method,which were normal group,asthma model group,Dioscorea nipponica Makino group,montelukast group,and Dioscorea nipponica Makino and montelukast group.Except the normal control group,the rats in each group were made into asthma models.The preparation method is divided into two stages of sensitization and challenge;on the 1st and 8th days,intraperitoneal injection of egg protein aluminum hydroxide solution 1ml(containing 10 mg of egg protein and 200 mg of aluminum hydroxide)for sensitization;from the 15 th day on asthma model group,and the pendant dragon Rats in the Dioscorea nipponica Makino group and the Dioscorea nipponica Makino groups were aspirated by inhalation of 2%egg protein(OVA),and the amount of atomization was medium.Inhaled naturally for30 minutes,once a day for 20 consecutive days.The normal control group used physiological saline instead of egg protein aluminum hydroxide solution and 2% OVA for sensitization and challenge.The rats in each group were given corresponding drug treatment 2 h before each atomization.The normal group and the asthma model group were administrated with normal saline,and each rat was administrated with 1ml.Samples were taken after 20 days of continuous gavage challenge.Light microscope was used to observe the pathological changes of lung tissue in each group;the levels of interleukin-5(IL-5)and cysteine leukotriene(Cys LTs)in the serum of each group were detected by enzyme-linked immunosorbent assay(ELISA);Reverse transcription polymerase chain reaction(RT-PCR)was used to detect the m RNA expression levels of Cys LTs receptor 1 and Eot-3 in rat lung tissues.Result 1.General performance:In the normal group,from the first day of the experiment to before the material was taken,the mental state of the rats was better than the rats in the other groups.The reaction speed was quick,the breathing was stable,the food and drinking conditions were good,the coat color was shiny,and the weight gradually increased.Rats in the model group began to suffer from mental insufficiency from day 7 and began to suffer from poor mental state on day 14,slow response,shortness of breath,nodding their noses,slow movements,abdominal cramps,huddled together,unstable standing,and erratic fur There is no luster,and the amount of water intake and drinking is less than that in the normal group.There is a significant difference between the body weight changes of the rats before and after modeling and the normal group(P<0.05).Each treatment group continued to administer drugs for 20 days.The spirit gradually improved,the breathing gradually returned to steady,and the gloss and reaction speed of the fur were improved compared with before.2.Pathological conditions:(1)Normal group: The bronchial lumen was smooth,the epithelium was intact,the airway mucosa was edema-free,the alveolar septum was normal,and eosinophils were occasionally seen around the airways and blood vessels.(2)Asthma model group: bronchial and bronchiolar lumen stenosis,obvious hyperplasia of epithelial cells,thickening of smooth muscle,pulmonary interstitial edema,vascular dilatation and congestion around the airways,and surrounding tissues,alveolar walls and surrounding areas can be seen in the lung tissue of rats.Infiltration of inflammatory cells.(3)Each treatment group: only a small number of inflammatory cells were seen in the alveoli of the group,and only a small degree of edema appeared in some bronchial mucosa.The infiltration of bronchial wall and surrounding inflammatory cells was less than that in the model group.3.Detection of IL-5 and Cys LTs in serum:Compared with the normal group,the serum IL-5 and Cys LTs levels in the model group were significantly increased(P<0.05);compared with the model group,the serum IL-5 in the montelukast group,Dioscorea nipponica Makino and montelukast group,pangolin groups’ levels of Cys LTs were significantly reduced(P<0.05);compared with the montelukast group,the levels of IL-5 and Cys LTs in the serum of the Dioscorea nipponica Makino and montelukast group were significantly reduced(P<0.05),and the serum levels of the Dioscorea nipponica Makino group were no statistically significant difference between IL-5 and montelukast group(P<0.05).Serum Cys LTs level in montelukast group was lower than that of Dioscorea nipponica Makino group(P<0.05),the difference was statistically significant.4.Cys LTR1 m RNA and Eot-3 m RNA expression levels in lung tissue:Compared with the normal group,the levels of Eot-3m RNA and Cys LTR1 m RNA in the lung tissue of the model group were significantly increased(P<0.05);compared with the model group,the montelukast group,the Dioscorea nipponica Makino and the monstrut group,and the Dioscorea nipponica Makinos’ levels of Eot-3 m RNA and Cys LTR1 m RNA in the lung tissue of the rats in the group were decreased(P<0.05);compared with the montelukast group,the levels of Eot-3m RNA and Cys LTR1 m RNA in the lung tissue of the Dioscorea nipponica Makino and Montelukast groups were lower low(P<0.05),the difference was statistically significant;Cys LTR1 m RNA level in lung tissue of the Dioscorea nipponica Makino group was significantly higher than that of the montelukast group(P<0.05);Eot-3m RNA level in the lung tissue of the Dioscorea nipponica Makino group and montelukast There was no significant difference in the group(P<0.05).Conclusion 1.Dioscorea nipponica Makino has protective effect on lung tissue and the content of IL-5 and Cys LTs in the serum of asthmatic rats.2.Dioscorea nipponica Makino can significantly reduce the m RNA levels of IL-5,Cys LTs and Cys LTR1 and Eot-3 m RNA in the serum of asthmatic rats,and the effect of reducing IL-5 and Eot-3m RNA is comparable to that of montelukast.3.Dioscorea nipponica Makino can enhance the effect of montelukast in relieving asthma,which may be related to the inhibition of the Cys LTs-mediated inflammation pathway. |
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