Experiment And Study Of Shaoyao Decoction Combines With Dragon’s Blood On Acute UC Rat Models

Objective:To explore the pathogenesis of UC,Shaoyao decoction combines with Dragon’s blood therapeutic effect and possible mechanism of effect on acute UC rat models,in order to build the new pathogenetic theory of UC,new therapeutic targets,and provide a basis for the application of Shaoyao decoction combines with Dragon’s blood to clinic.Methods:62 healthy and clean SD rats,10 rats were randomly divided into blank group(group A),the remaining 52 rats drank 4% dextran sulfate(DSS)freely a week to set up acute UC model,after set up UC model successfully,the 52 rats were randomly divided into the model control group(group B),the western medicine group(group C),the Shaoyao decoction group(group D),the Dragon’s blood group(group E),and the composite(Shaoyao decoction + Dragon’s blood)group(group F).Then,treating them with drug for 2 weeks.And then draw blood and cup off colon tissues of the rats.Using ELISA to detect the expression of TNF-α and IL-1β in serum of rats,using IHC to detect the mean optical density of AQP3 and AQP4 in colon tissues of rats,and using Rt-PCR to detect m RNA expression of AQP3 and AQP4 in colon tissues of rats.Results:(1)After set up UC model successfully,the expression of serum TNF-α in group B,C,D,E and F increased significantly,the difference between group A and group B,C,D and E was statistically significant(P<0.05).After treated,the expression of serum TNF-α in group C,D,E and F decreased significantly,the difference between group B and group C,D,E and F was statistically significant(P<0.05),the difference between group F and group E was statistically significant(P<0.05),the difference between group F and group C and D with no statistically significant(P>0.05).(2)After set up UC model successfully,the expression of serum IL-1β in group B,C,D,E and F increased significantly,the difference between group A and group B,C,D,E and F was statistically significant(P<0.05).After treated,the expression of serum IL-1β in group C,D,E and F decreased significantly,thedifference between group B and group C,D,E and F was statistically significant(P<0.05),the difference between group F and group D and E was statistically significant(P<0.05),the difference between group F and group C with no statistically significant(P>0.05).(3)After set up UC model successfully,the mean optical density of AQP3 in colon tissues in group B,C,D,E and F decreased significantly,the difference between group A and group B,C,D and E was statistically significant(P<0.05),the difference between group A and group F with no statistically significant(P>0.05).After treated,the mean optical density of AQP3 in group C,D,E and F increased significantly,the difference between group B and group C,D,E and F was statistically significant(P<0.05),the difference between group F and group C,D and E was statistically significant(P<0.05).(4)After set up UC model successfully,the mean optical density of AQP4 in colon tissues in group B,C,D,E and F decreased significantly,the difference between group A and group B,D and E was statistically significant(P<0.05),the difference between group C and F with no statistically significant(P>0.05).After treated,the mean optical density of AQP4 in group C,D,E and F increased significantly,the difference between group B and group C,D,E and F was statistically significant(P<0.05),the difference between group F and group D and E was statistically significant(P<0.05),the difference between group F and group C with no statistically significant(P>0.05).(5)After set up UC model successfully,the expression of AQP3 m RNA in colon tissues in group B,C,D,E and F decreased significantly,the difference between group A and group B,C,D,E and F was statistically significant(P<0.05).After treated,the expression of AQP3 m RNA in group C,D,E and F increased significantly,the difference between group B and group C and F was statistically significant(P< 0.05),the difference between group B and group D and E with no statistically significant(P>0.05),and the difference between group F and group E was statistically significant(P<0.05),the difference between group F and group C and D with no statistically significant(P>0.05).(6)After set up UC model successfully,the expression of AQP4 m RNA in colon tissues in group B,C,D,E and F decreased significantly,the difference between group A and group B,D and E was statistically significant(P<0.05),the difference between groupA and group C and F with no statistically significant(P>0.05).After treated,the expression of AQP3 m RNA of group C,D,E and F increased significantly,the difference between group B and group C,D,E and F was statistically significant(P<0.05),the difference between group F and group D and E was statistically significant(P<0.05),the difference between group F and group C with no statistically significant(P>0.05).Conclusion:Shaoyao decoction combines with Dragon’s blood can decrease the serum expressions of TNF-α and IL-1β of UC rat models,increase the level of mean optical density of AQP3 and AQP4 in colon tissues as well as the expression of m RNA of AQP3 and AQP4 in colon tissues,it suggesting that these may be the mechanism of effect of UC.