| Objectives Establish an intermittent hypoxia rat model to simulate the pathogenesis of Obstructive sleep apnea hypopnea syndrome(OSAHS),observe the weight gain of rats,nucleic acid load of major bacterial components in the intestine,blood oxidative stress index levels Changes,and assess the degree of obesity,the occurrence of intestinal bacterial translocation,and the degree of intestinal mucosa and mesenteric lymph node damage or apoptosis in rats,and explore the effects of intermittent hypoxia on changes in body weight gain and changes in the intestinal microecological environment in rats Impact.Methods Forty adult male Wister rats were selected for routine feeding for 1 week,and were randomly divided into a control group(UC group)and an intermittent hypoxic group(IH group).Both groups of rats were treated in accordance with the requirements of their respective experiments.After successful modeling,on the last experimental day of 2,4,6,and 8 weeks after the start of the experiment,the rats of each group were anesthetized with 20% urethane 5ml /100 g to weigh the body weight and body length,and Calculate Lee’s index,body weight gain and body weight growth rate;aseptically take mesenteric lymph node grinding fluid for anaerobic culture to evaluate intestinal bacterial translocation;aseptically extract genomic DNA of intestinal contents for q RT-PCR detection to determine gas-producing pods Clostridium perfringens,Escherichia coli,Bacteroides,Bifidobacterium,Lactobacillus and other intestinal bacterial component DNA load;HE staining to observe changes in mesenteric lymph nodes and intestinal tissue structure;ELISA method to measure serum diamine oxidase in central venous blood(Diamine oxidase,DAO),Superoxide Dismutase(SOD),Malondialdehyde(MDA),and Reactive Oxygen Species(ROS);Immunohistochemical staining was used to detect the expression levels of Bax and BCl-2,and to evaluate the degree of mesenteric lymph node apoptosis The data obtained are all expressed in x ± s,SPSS 20.0 software was used for statistical analysis,and the mean comparison between multiple groups was performed using one-way analysis of variance;correlation analysis was performed using pearson correlation analysis;Two-way analysis of variance,P <0.05 was considered statistically significant.Results 1 Dynamic monitoring of blood gas analysis in rats found that the levels of Pa O2 and Sa O2 in the IH group were significantly lower than those in the UC group,and the difference was statistically significant(P<0.05),indicating that the animal modelling was successful.2 There was no significant change in body weight gain at all stages in the UC group.The degree of obesity in the IH group was more serious than that in the UC group,but the body weight gain at each time point gradually decreased with time,and the early changes were more obvious.3 There were no significant fluctuations in SOD,MDA,ROS,DAO and other indicators of rats in UC group at all stages(P>0.05);IH group rats: from week 2 to week 8,SOD activity level showed a gradual downward trend(P<0.05);MDA,ROS,DAO and other indicators have gradually increased.4 The degree of intestinal mucosal barrier damage,mesenteric lymph node morphological damage and cell apoptosis in IH group were progressively increased,but no significant change was observed in UC group.5 There was no obvious abnormality in the culture of rat mesenteric lymph node grinding fluid at all time points in the UC group.The IH group had intestinal bacterial translocation since the fourth week and later,and was identified as Clostridium perfringens.6 The level of nucleic acid load of main bacterial bacteria in intestine of rats in UC group changed only slightly or was basically the same,while the average load of nucleic acid load of main bacterial bacteria in intestine of rats in each stage of IH group changed significantly at each time point.Conclusions 1 Under intermittent hypoxic conditions,the degree of obesity in rats became more and more serious.During the experimental observation period,the weight gain increased,and the early increase was more obvious.2 Intermittent hypoxia can cause oxidative stress damage to the rat body,the balance of the intestinal microecological environment is broken and intestinal bacterial translocation occurs.Figure14;Table14;Reference 119... |
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