Effect Of Lingguizhugan Decoction Combined With Pidotimod On The Expression Of TSLP、TNF-α、VCAM-1 And IL-4 In Rats With Spleen Deficiency And Wet-filled Allergic Rhinitis

Objective: The protein expression of TSLP、TNF-α、VCAM-1 and IL-4 in serum and m RNA in nasal mucosa were studied by Lingguizhugan Decoction and Lingguizhugan decoction combined with pidotimod To explore the effect of Lingguizhugan Decoction and Lingguizhugan decoction combined with pidotimod on allergic rhinitis in rats with spleen deficiency and humid,and to provide an objective basis for the treatment of variant rhinitis.Methods:Wistar rats were randomly divided into 9 groups,10 in each group.They were blank group,spleen deficiency and wet-filled allergic rhinitis model group,Lingguizhugan decoction(high,medium and low dose)group,Lingguizhugan decoction(high,medium and low dose)combined with pidotimod group,dexamethasone group.The blank group did not do any treatment,the other groups all took the intraperitoneal injection of ovalbumin aluminum hydroxide mixture,and 4℃ Senna liquid into the stomach to establish a rat model of spleen deficiency and humid rhinitis.Serum and nasal mucosa were collected after 6 weeks of treatment.The expression of TSLP、TNF-α、VCAM-1 and IL-4 in serum was detected by ELISA,and the expression of TSLP、TNF-α、VCAM-1 and IL-4 m RNA in nasal mucosa was detected by RT-q PCR.Results: The symptom score of the model group and the treatment group was more than 5,and the score of the blank group was less than 5,The difference was statistically significant(P < 0.05).After 4℃ senna liquid gavage for two weeks,the rats’ spleen deficiency and wet-filled type symptom also appeared loose stools,sticky and greasy stools in addition to sneezing,runny water and scratching nose.Explain that spleen deficiency and wet-filled type rhinitis model was succeed.in the results of the rat PCA experiment,the diameter of skin hardening and dye diffusion in themodel group were all larger than 10 mm,the PCA reaction was positive,and the PCA reaction in the blank group was negative.The successful model of allergic rhinitis can be further judged.The relative factors in serum and nasal mucosa were detected by Elisa and RT-q PCR.Compared with the blank group,TSLP、TNF-α、VCAM-1 and IL-4 in the model group increased in different degrees(P <0.05).After medication,the expression of TSLP、TNF-α、VCAM-1 and IL-4 in each treatment group was inhibited in different degrees compared with the model group(P <0.05).Among them,the combined drug group,Lingguizhugan decoction group and dexamethasone group decreased significantly,and the effect of low dose group of Lingguizhugan decoction was relatively poor.The trend of expression of related factors in Elisa and RT-q PCR was about the same.Conclusions:The expression of TSLP、TNF-α、VCAM-1 and IL-4 gene and protein were significantly increased in rats.The nasal symptoms of rats were obvious.The gene and protein expression of related factors decreased after the intervention,and the nasal symptoms of rats were relieved.This rusults indicating that lingguizhugan decoction and lingguizhugan decoction combined with pidotimod can alleviate the symptoms of spleen deficiency and wet-filled allergic rhinitis by inhibiting the expression of TSLP、TNF-α、VCAM-1 and IL-4 in nasal mucosa and serum of allergic rhinitis rats,and achieve the therapeutic effect.