Establishment Of Transplanted Tumor Models In Different Parts Of NSCLC

Objective:NSCLC A549 cells carrying luciferase reporter gene were used to establish animal models of NSCLC subcutaneous,peritoneum and lung transplantation tumor at the same time.Methods:NSCLC A549 cells carrying luciferase reporter gene were injected into the tail vein,abdomen and subcutaneous of nude mice to establish lung transplantation tumor model,peritoneal transplantation tumor model and subcutaneous transplantation tumor model.Observe the general condition of the model,and detect the tumorigenesis by bioluminescence one week later.Four weeks later,the tumor formation and metastasis of NSCLC A549 cells were observed by bioluminescence and ¹⁸F-FDG PET-CT.The results of the two methods are compared and analyzed.Results:The tumorigenesis rate of subcutaneous and peritoneum transplanted tumor models was 100%,that of lung transplanted tumor models was 60%.Bioluminescence detection detected fluorescence signals in subcutaneous,abdominal and lung of animal models.¹⁸F-FDG PET-CT.detected abnormal hypermetabolism signal under the skin,scattered abnormal hypermetabolism signal in the abdomen,and hypermetabolism signal in the lung,but the metabolic boundary between ¹⁸F-FDG PET-CT.and normal lung tissue was unclear.Conclusion:It is safe and reliable to establish lung transplantation tumor model,peritoneal transplantation tumor model and subcutaneous transplantation tumor model by injecting tumor cells through tail vein,abdomen and subcutaneous site.Bioluminescence detection is accurate and sensitive in the detection of tumor formation in animal models.¹⁸F-FDG PET-CT can locate the tumor tissue in the normal low metabolism area and provide the metabolic activity of tumor cells.Objective: To detect the expression of HIF-1 and Pimo in NSCLC A549 cell transplanted subcutaneously,and to verify the presence of hypoxia in NSCLC.The expression of two kinds of hypoxia markers in NSCLC was compared and the correlation was analyzed.Methods: The expression of HIF-1 and Pimo in NSCLC A549 cells was detected by immunofluorescence technique.The correlation between HIF-1 and Pimo expression was analyzed by Pearson linear correlation analysis,and the difference between HIF-1 and Pimo expression was analyzed by t-test of two independent samples.Result: 1.HIF-1 and PIMO were widely expressed in NSCLC A549 cell transplanted tumor tissues,and the expression around the tumor necrosis area was significantly enhanced.2.The expression of HIF-1 was positively correlated with that of PIMO（r = 0.712,P <0.05）.3.The expression of PIMO was significantly higher than that of HIF-1（P = 0.001 <0.05）Conclusion: 1.There are a wide range of hypoxic areas in NSCLC tumor tissue,especially in necrosis area.2.The expression of HIF-1 was positively correlated with that of PIMO in NSCLC.3.The expression of PIMO was stronger than that of HIF-1 in NSCLC with the same degree of hypoxia.