Application Of Molecular Imprinting Technique And Network Pharmacology In The Research Of Traditional Chinese Medicine

Traditional Chinese medicine(TCM)plays a therapeutic role under the guidance of "traditional Chinese medical theory".However,it has been the great difficulty to extract and purify medicinal substances,and to elucidate the complex mechanism.As the development of scientific thought and the innovation of technology,molecular imprinting technology(MIT),which possesses the predetermination,highly recognizability and practicability,can realize the separation,purification and concentration of active ingredients from TCM.At the same time,with the big data era,the use of network pharmacology can provide a theoretical basis to elucidate the mechanism of TCM with many ingredients and targets.In this paper,tanshinone IIA molecular imprinted polymers(MIPs)were prepared by precipitation polymerization method,and the obtained MIPs were used in the study of serum pharmaceutical chemistry.Meantime,the network pharmacology was used to investigate the possible mechanism of Zaoren anshen prescription(ZRASP)in the treatment of insomnia.Following is the research:1.Tanshinone IIA,methacrylic acid(MAA),ethylene glycol dimethacrylate(EGDMA)and 2,2-azobisisobutyronitrile(AIBN)were respectively used as the template molecule,functional monomer,crosslinking agent and initiator to prepare Tanshinone IIA MIPs.The obtained tanshinone IIA MIPs were found to have good dispersivity and uniform particle size with the help of field emission scanningelectron microscope(SEM).Dynamic adsorption test,static adsorption test and selective adsorption test showed that tanshinone IIA MIPs reached adsorption equilibrium in about 3 h,with a maximum adsorption capacity of 34.09 μmol/g,and a specific selectivity to tanshinone IIA.Finally,efficient separation and sensitive detection of metabolites in serum were realized by MIPs coupled with solid phase extract(MIPs-SPE).2.Zaoren anshen prescription(ZRASP)is composed of Ziziphi spinosae semen,Salvia miltiorrhiza bunge and Schisandrae chinensis fructus,which is a commonly prescription used for insomnia.In the network pharmacology research of ZRASP,the potential active components of ZRASP were collected through the Traditional Chinese Medicine systems pharmacology database and analysis platform(TCMSP)and the national scientific data sharing platform for population and health pharmaceutical information center.Then potential active ingredients of ZRASP for certain targets were acquired through TCMSP and Herbal ingredients’ targets database(HIT).The information related to relevant targets of insomnia was collected by Therapeutic target database(TTD),Drug Bank,Online Mendelian inheritance in man(OMIM),Comparative toxicogenomics database(CTD)and Human gene database(Gene Cards).The tool "Venny 2.1" was used to map the potential target of the potential active ingredients from ZRASP to the relevant target of insomnia,so as to obtain the target of ZRASP for insomnia treatment.Cytoscape software was used to draw the network map of "active ingredient-action target",and the The protein-protein interaction and the action target type of ZRASP for insomnia treatment were obtained respectively through STRING and Dis Ge NET database.Cluster Profiler was used to conduct Gene ontology(GO)on the target,and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis was conducted on thetarget through the Clue GO and Clue Pedia in Cytoscape.The results of Network pharmacology showed that ZRASP has 67 main active components and 73 targets involving in the treatment of insomnia.The targets are mostly receptors,enzymes,signal molecules and other substances,and GO enrichment is high in the activity of neurotransmitter receptors,G protein-coupled amine receptors and ammonium ion binding,and there are 47 significant KEGG pathways.