Study On Holographic Microscopic Imaging Technique For Cancer Cells

Digital holography is a new microscopic imaging technology combining the photoelectric detection technology and computer technology based on the principle of optical holography.Compared with conventional microscopies,it offers a more advantageous approach of non-contract,label-free,non-destructive,faster and quantitative three-dimensional imaging using a single hologram.In recent years,holographic imaging applications of biological cells have attracted scientists’ much attention because of the unique imaging advantages of digital holography and its great imaging potential for imging biological cells.Thus,it has been widely used to observe biological cells espically cancer cells in the fields such as the three-dimensional morphological measurement,biological parameter analysis and real-time morphological minitoring which provides help for the diagnose and treament of cancer.This thesis first introduces the basic working principle of digital holography,and then chooses the in-line holographic recording system after considering the advantages and disadvantages of several holographic recording methods.Based on the digital in-line holography,we design and build the in-line holographic microscope,and calibrate its basic system parameters by USAF1951 resolution chart and other tested samples.Through the calibration,it is demonstrated that the system provides higher lateral resolution and larger magnification than the common in-line holographic microscopes.Finally,we carry out a series of holographic imaging test experiments on biological cells which demonstrate the system capablity of morphological imaging,biological parameter analysis and living cells’ imaging.Next,in this work,the developed in-line holographic microscope is used in holographic microscopic imaging study of cancer cells.Firstly,the three-dimensional morpholography and biological parameters of different cancer cells are measured and analyzed.Then,holographic imaging of SMMC-7721 liver cells during one growth period and after the drug treament is studied.The liver cancer cell growth and the drug response are qualitatively and quantitatively studied using the information of the three-dimentional morphological changes.Finally,the real-time holographic monitoring of living A549 lung cancer cells is implemented by recording dynamic changes of three-dimensional morphology of lung cancer cells during a period of time.