Expression And Significance Of SP1,KLF4 And P21 In Epithelial Ovarian Cancer

Objective:To analyze the abnormal expression of SP1,KLF4 and P21 in epithelial ovarian cancer and their correlation with clinicopathological features of epithelial ovarian cancer,so as to find effective early diagnostic and therapeutic evaluation indicators for epithelial ovarian cancer.Methods:48 patients with epithelial ovarian cancer who underwent gynecological resection in our hospital from June 2011 to August 2015 were collected.In addition,30 cases of benign ovarian tumor,30 cases of borderline tumor(15 cases of serous tumor and 15 cases of mucinous tumor)and 40 cases of normal tissue samples of hysteromyoma treated with hysterectomy plus bilateral appendectomy were collected as control.The positive expression of SP1,KLF4 and P21 was detected by immunohistochemistry.The relationship between SP1,KLF4 and P21 and clinicopathological characteristics was analyzed;and analyze the influence of three factors on the prognosis of patients with epithelial ovarian cancer Results:(1)The positive rate of Sp1 in epithelial ovarian cancer(72.9%)was significantly higher than that in normal ovarian tissue(7.5%),benign ovarian tumor(6.7%),borderline ovarian tumor(13.3%)(P < 0.05);the positive rate of KLF4 in epithelial ovarian cancer(29.2%)was significantly lower than that in normal ovarian tissue(87.5%),benign ovarian tumor(86.7%),borderline ovarian tumor(80.0%)(P < 0.05);The p21 positive rate of epithelial ovarian cancer(25.0%)was significantly lower than that of normal ovarian tissue(67.5%),benign ovarian tumor(66.7%),borderline ovarian tumor(60.0%)(P < 0.05).(2)Spearman grade correlation analysis showed that SP1 was negatively correlated with the expression of KLF4 and P21(r=-0.519,-0.481,P<0.01);KLF4 was positively correlated with the expression of P21(r=0.462,P<0.01).The positive expression rate of SP1 in stage III-IV was significantly higher than that in stage I-II(P<0.05),and the positive expression rates of KLF4 and P21 were lower than those in stage I-II(P<0.05);with the decrease of differentiation degree,the positive expression rate of SP1 gradually increased(P<0.05),the positive expression rates of KLF4 and P21 gradually increased(P<0.05);thepositive expression rate of SP1 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis(P<0.05),and KLF4 gradually increased(P<0.05).The positive expression rate of P21 was lower than that of patients without lymph node metastasis(P <0.05).(3)Kaplan Meier method was used to analyze the survival of patients.The results showed that the survival time of patients with positive expression of SP1,KLF4 and p21 was10-60 months(median time 40 months),30-65 months(median time 59 months),30-65months(median time 60 months);the survival time of patients with negative expression of SP1,KLF4 and p21 was 25-65 months(median time 60 months),10-52 months(median time 40 months),10-52 months(median time 40 months).The survival time of patients with positive expression of SP1 was significantly shorter than that of patients with negative expression(P < 0.05);the survival time of patients with positive expression of KLF4 and p21 was significantly longer than that of patients with negative expression(P < 0.05).Conclusion:(1)Compared with normal ovarian tissue,benign ovarian tumor and borderline ovarian tumor,the positive expression of Sp1 protein in epithelial ovarian cancer was significantly higher,and the expression of KLF4 and p21 protein was significantly lower.(2)The expression of SP1 was negatively correlated with the expression of KLF4 and P21,and positively correlated with the expression of P21 in epithelial ovarian cancer.(3)The expression of SP1,KLF4 and P21 was related to the pathological stage,differentiation degree and lymph node metastasis of epithelial ovarian cancer.(4)The abnormal increase of Sp1 protein expression and the down-regulation of KLF4 and p21 protein expression will shorten the survival time of ovarian cancer patients.Therefore,the detection of their expression level is conducive to the early diagnosis and prognosis evaluation of ovarian epithelial cancer.SP1,KLF4 and p21 genes may also be new targets of ovarian cancer treatment.