Correlation Between COX-2 Gene Rs689466 Loci And Aspirin Resistance In Ischemic Stroke In Han Population In Northeast China

Objective:Platelet aggregation plays a key role in the occurrence of ischemic stroke.Although patients take aspirin for anti-platelet aggregation treatment,patients still have recurrent ischemic stroke.With the in-depth study of genetic genes,it has been found that genetic factors play an important role in aspirin resistance.The genetic susceptibility genes for aspirin resistance were screened out to achieve individualized aspirin treatment.This article aims to explore the relationship between polymorphism of rs689466 COX-2 gene and aspirin resistance in patients with ischemic stroke in the Northeast Han population.Methods:A total of 146 patients with cerebral infarction from the Han population in the Northeast were included.All patients were given aspirin orally.The platelet aggregation rate was measured by optical method and the improved multiple multiplex ligation detection reaction(IMLDR)was used for genotyping.ADP-induced mean platelet aggregation≥70%or(and)AA-induced mean platelet aggregation≥20%as aspirin resistance(AR)group,neither of which is eligible for aspirin-sensitive(AS)group.SPSS24.0 statistical software was used for statistical analysis,and the frequency distribution difference between the two groups of the target genotype and allele was analyzed by?~2 tests to explore the relationship between the COX-2 gene rs689466 and aspirin resistance.Results:The detection of the genotype of the rs689466 locus polymorphism of the COX-2 gene in the aspirin sensitive group by Hardy-Weinberg genetic balance law showed that the population in this study was in line with Hardy balance and had a good representativeness.Among the 146 patients with cerebral infarction,the incidence of aspirin resistance was 33.6%(49/146).The age of patients in the aspirin resistance group was significantly higher than that of the aspirin sensitive group.Multi-factor analysis indicated that advanced age was a risk factor for aspirin resistance.The CC genotype in the aspirin resistance group at the rs689466 locus of the COX-2 gene was significantly higher than that in the aspirin sensitive group(P<0.05),but the frequency of the low-frequency allele C at the rs689466 locus of the COX-2 gene was not statistically different.The model was constructed again and found to be statistically significant between the two groups under the dominant model condition(P<0.05).After applying binary logistic regression to correct the related risk factors,the study found that the COX-2 gene rs689466allele C was more likely to cause aspirin resistance(OR=3.634,95%CI:(1.350,9.787),P=0.011).Conclusion:The allele C of the rs689466 locus of the COX-2 gene may be related to aspirin resistance in the Han population in Northeast China.