Effect Of Lactobacillus T3L On Browning Of Adipose Tissue In High-fat Diet Mice

Excessive triglycerides in high-fat and high-sugar diets can lead to excessive accumulation of white adipose tissue in the body,leading to obesity.In recent years,Lactobacillus,as a kind of regulation of microbiota balance to alleviate or prevent obesity and related metabolic diseases caused by diet,has become the focus of attention.Many studies have shown that probiotics can also stimulate brown adipose tissue activity,promote the browning of white fat,and regulate host metabolic homeostasis and energy balance.However,the mechanism of Lactobacillus inhibiting obesity is still not well studied.Therefore,probiotics,which enhance the thermogenesis of brown fat and promote the Browning of white fat,have gradually become a new and potential treatment strategy for obesity.In this study,a high-fat diet was adopted to construct an obesity model,and Lactobacillus T3 L and LGG were administered by gavage,mainly to investigate the effect of L.Coryniformis subsp.torquens T3 on the diversity of gut microbiota.Research Lactobacillus regulation of brown fat,stimulate the BAT cell activity and promote the white fat Browning mechanism by the form of the main control signal pathway enrichment.Thereby inhibiting fatty deposits and prevent obesity,aims to clear the intestinal microbes and the connection between the brown fat is changed,prevention and treatment of obesity for subsequent probiotics to provide new train of thought and theory basis.The intervention of Lactobacillus T3 L could significantly reduce the body weight increment,body fat rate and serum related indexes in obese mice.Morphological results showed that Lactobacillus T3 L could effectively improve fatty deposition in liver and increase the content of multicellular adipocytes in adipose tissue.Analysis of serum LPS and TNF-α showed that Lactobacillus T3 L had a good regulatory effect on adipocytes and reduced chronic infl ammation of adipocytes.Fluorescence quantitative PCR analysis of the marker genes of browning in adipose tissue showed that Lactobacillus T3 L could up-regulate the expression levels of key genes such as UCP1 and PGC-1α in brown fat cells.The expression of upstream coregulators PRDM16,PPARγ and SIRT6 in different pathways was also increased,stimulating BAT cell activity.Meanwhile,transcription factors ASK1,UCP1 and SIRT6,which are the same as those of brown fat,were also expressed in white adipose tissue.This indicates that Lactobacillus T3 L can simultaneously regulate the heat-producing core genes of white and brown fat,activate the regulatory pathway,and then make white fat cells show the phenotype of beige fat and promote the Browning of fat.In addition,PCR results also showed that Lactobacillus T3 L could effectively improve the integrity of the structure of the tight junction protein in the intestinal tissue of mice on a high-fat diet,so as to protect the intestinal barrier system of mice and reduce the expression of serum LPS and the production of adipocyte inflammatory factors.The diversity and relative abundance of intestinal flora in mice were analyzed by 16 S r DNA sequencing.Lactobacillus T3 L can improve the intestinal flora structure disorder of mice caused by high-fat diet and increase the relative abundance of short-chain fatty acid bacteria in the intestinal tract,including Streptococcus,Blautia,Bacteroides,Romboutsia and Faecalibaculum.Changes in the structure of intestinal microflora are correlated with Browning of adipose tissue.The dry of Lactobacillus T3 L can improve the absorption of triglycerides and cholesterol in the gastrointestinal tract by regulating the balance of intestinal microflora,thus reducing the proportion of lipids entering the host blood.More notably,intestinal microbial metabolites SCFAs,by binding to free fatty acid receptors FFAR2 and FFAR3,stimulate the expression of brownish marker gene PGC-1α,thereby increasing the biosynthesis of beige adipocytes and mitochondria and promoting adipogenic heat.The above experimental results are intended to provide a basis for other studies on the mechanism of probiotics regulating intestinal flora and affecting the regulation of fat browning,as well as provide new ideas for the prevention and treatment of obesity syndrome.