Significance And Expression Of Long Non-coding RNA MEG3 In Plasma Of Patients With Type 2 Diabetes Mellitus

Background: Diabetes mellitus(DM)is a set of caused by more etiology of metabolic disease characterized by chronic hyperglycemia,is caused by a lack of insulin secretion relative or absolute.In recent years,the incidence of diabetes has been increasing year in word,among which type 2 diabetes accounts for more than 90%.It has become one of the chronic non-communicable diseases that affect the health of people all over the world.Some literatures have pointed out that the pathogenesis of type 2 diabetes is complex,and insulin resistance is generally believed to be the main pathogenesis of type2 diabetes.Recent studies have demonstrated that long non-coding RNA(lnc RNA)play an important regulatory role in the development of insulin resistance.Therefore,exploring the correlation between lnc RNA and type 2 diabetes provides a new idea for the treatment of type 2 diabetes.More than 90% of the DNA in the human genome sequence can be transcribed,and only 1% to 2% of transcription product can encode proteins,and most of the transcript for lnc RNA.lnc RNA is located in the nucleus or cytoplasm,in the form of RNA regulation of expression in variety of levels,widely involved in the body’s physiological and pathological and pathological process,the vast majority of closely associated with many diseases clinically.It has been reported in the literature that a variety of lnc RNA have been found to be abnormally expressed in diabetic patients.The human maternal-expressed gene 3(MEG3)is located on human chromosome 14q32 and is a single-copy imprinted gene composed of 10 exons,which is strictly regulated by the imprinting control region(ICR).In recent years,with the continuous development and maturity of the new generation of sequencing technology,the expression level of lnc RNA MEG3 has been confirmed to increase significantly in liver tissues of diabetic mice compared with normal mice.After the deletion of MEG3 from primary liver cells isolated from high-fat fed mice,impaired glucose tolerance and glycogen synthesis have been improved.Therefore,the study on the differential expression of MEG3 in type 2diabetes patients and normal people provides theoretical support for the treatment of diabetes patients and early screening of susceptible populations.Objective: In this study,by detecting the expression level of lnc RNA MEG3 in the plasma of type 2 diabetes patients and healthy people,and combining with the Graphpad Prism8.0 statistical software for analysis and mapping,it was confirmed that the expression of lnc RNA MEG3 in type 2 diabetes patients was significantly down-regulated.Combined with the area under ROC curve,lnc RNA MEG3 has a certain diagnostic efficacy in type 2 diabetes,and may become a new potential biological marker for the diagnosis and disease assessment of type 2 diabetes.This finding provides new ideas and theoretical basis for early screening and treatment of type 2 diabetes.Methods: This study uses the case-control research method.The case sample were selected from the fourth affiliated hospital of China medical university endocrinology and metabolism department and obtain definite diagnosis of 20 cases of patients,to select in the center of the medical examination of healthy examination people as control.Venous blood,general data and biochemical parameters were collected from all subjects.This study was approved by the ethics committee of China medical university,and the subjects signed the informed consent for the collection of biochemical data and blood samples,and complied with the relevant regulations of ethics.In this study,the BIGO free RNA extraction kit was used to extract the RNA from the plasma,and then the extracted RNA was reversely transcribed into c DNA.Real-time PCR was used for amplification and the expression level of lnc RNA MEG3 was detected.Graphpad Prism 8.0 statistical software was used to analyze and graph the data.The measurement data conforming to normal distribution were expressed as Mean+ standard deviation(Mean+SD),and the difference between two independent sample groups was compared by t test.The measurement data that did not conform to the normal distribution were represented by median or quartile spacing,and the difference between the two independent sample groups was compared by mann-whitney U rank sum test.The predictive/diagnostic value of type 2 diabetes was analyzed by ROC curve method.P <0.05 was used as the criterion with statistical significance.To investigate the expression and significance of lnc RNA MEG3 in type 2 diabetes mellitus.Results: The expression level of lncRNA MEG3 in plasma of the type 2 diabetes group was significantly lower than that of the healthy control group(0.302 times of the normal level),with statistically significant difference(P<0.05).The receiver operating characteristic curve(ROC)curve of the enrolled population was made,and the area under the curve of lnc RNA MEG3 was 0.790,with a 95%CI of 0.578-1.000(P<0.05).The sensitivity and specificity were 66.7% and 88.9%,respectively,and the yoden index was 0.556.Conclusion:In this study,it was found that the expression of lnc RNA MEG3 in plasma of type 2 diabetes patients was significantly down-regulated,which may become a new potential biomarker for the diagnosis and condition assessment of type 2 diabetes.