| Objective:Through observing the level of interleukin-17A(IL-17A)secrated by T help 17 cell(Th17)of inflammatory bowel diseases(IBD)young rats model after feeding eclusive enteral preparation,Peptison,we discussed the enteral nutrition’s regulative effects on Th17 cells and revealed partial mechanism of EEN’s anction on IBD in children.Research method:Forty male Sprague-Dswley(SD)young rats were divided into control group,EEN group,IBD group and EEN-IBD group at random.Rats in the IBD group were given enemas using 2,4,6 trinitrobenzenesulfonic acid-ethanol(TNBS)solution for modeling,and rats in the control group were given enemas using normal saline(NS).And then these rats would had a normal diet for 7 days.Rats in the EEN-IBD group were given enemas using TNBS-ethanol solution for modeling,and rats in the EEN group were given enemas using NS,after which the two groups were fed with enteral nutrition(Peptison)for 7 days.After successful modeling,we recorded all rats’ general state,weights,stool property and occulted blood situations every day.Also,we assessed the disease activity index(DAI)fraction.After one week of intervention,we killed all rats and collected colon tissue and serum samples.We observed the pathological conditions of intestinal wall and mucosa after Hematoxylin-eosin(HE)staining,the expression of IL-17 A in serum detected by ELISA and expression of IL-17 A in intestinal tissue detected by Western blot and real-time PCR.Result:(1)General state:In IBD group and EEN-IBD group,diarrhea and bloody stool occurred immediately after modeling,followed by eating less,losing weight,listlessness,and messy mouse hair.After five days of EEN,stool property and occulted blood situations of rats in EEN-IBD group were apparently improved contrasted with those in IBD group.The other two groups’ mice with neat and shiny hair had a healthy diet and they rowed yellow or hunter green normal stool with no bloody stool.(2)Weight: After seven days,there was no significant difference between IBD group and EEN-IBD group about the growth rate of weight(P≥0.05).The growth rate of EEN group(51.29±3.61)% was under that of the control group(60.17±9.32)%,and the difference was obviously significant in the statistical field(P<0.05).(3)DAI score:The disease activity index score of IBD group and EEN-IBD group decreased day by day.On the seventh day,compared with EEN-IBD group the score of IBD group showed an obviously statistical increasing(P<0.05).The score of control group and EEN group were zero.(4)Gross tissue and pathology:In the IBD group,gross tissue and pathological section showed changes in inflammation such as intestinal wall congestion,edema and ulceration,etc.Under the microscopic,we observed the destruction of the intestinal epithelium,decreased glands,and a lot of inflammatory cells in the submucosa.In the EEN-IBD group,intestinal inflammation,mucosal hyperemia and edema were mild.The pathology showed a small amount of inflammatory cell infiltration in the mucosa and the glandular structure was mildly disturbed.The other two groups had no abnomality.(5)Expression of IL-17A:On the seventh day,compared with EEN-IBD group and Control group,IL-17 A expression in serum and intestinal tissues group of IBD group showed an obviously statistical increasing(P<0.05).No significant difference was noted in the expression of IL-17 A among EEN-IBD group、control group and EEN group(P≥0.05).Conclusion:EEN can effectively reduce the intestinal immune inflammatory response in IBD young rats,and one of its mechanisms might be to regulate Th17 cells,mainly reduce the expression of the cytokine IL-17 A in the intestinal mucosa,and thus reduce the aggregation and infiltration of related inflammatory factors. |
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