Study On Cell Membrane Permeability Of Mouse Oocytes

Objective Permeability of oocytes play an important role in the optimization of their cryopreservation.However,there are still no systematical investigations on oocyte transport properties from the viewpoint of single-cell trapping and high precision perfusion,especially with the powerful microfluidic approach.To design and fabricate a microfluidic device that automatically captures a single oocyte and systematically studies the permeability of oocytes.Methods Using a microfluidic device with a unique microcolumn array for trapping oocytes,microfluidic chips were placed under a microscope and mice oocytes were injected into microchannels.Three different concentrations of CPA(0.5M,1.0M and1.5M EG)were selected for multi-step and single-step CPA addition schemes and parameters were compared using 2-p model.Results This novel microfluidic device is safe for oocytes.It indicates that the Lp estimated from multi-step is significantly different from the single-step of 0.5 M and 1.0M EG,while no significant difference with 1.5 M EG.Conclusion This novel microfluidic device is safe for oocytes.The transport characteristic parameters measured by single step osmotic displacement cannot be used to predict the osmotic response of multi-step CPA to oocytes.