The Expression Of Voltage-gated Sodium Channel Subtype Nav1.5 In Oral Squamous Cell Carcinoma Cells And Its Relationship With In Vitro Cell Metastasis

Objective At present,oral squamous cell carcinoma accounts for a high incidence of head and neck cancers and seriously affects the lives and health of patients.The expression of voltage-gated sodium channels in epithelial-derived metastatic cancer cells is often higher than that of homologous normal cells,and its expression is positively correlated with the invasion and metastasis of cancer cells.Therefore,this study was to observe the expression of Nav1.5 in oral squamous cell carcinoma SCC3 cells and its relationship with the proliferation and invasion of oral squamous carcinoma cell SCC3 in vitro.Methods The inhibitory effect of TTX on the proliferation of oral cancer cells was detected by MTT assay;the effect of TTX on the invasive ability of oral squamous cell carcinoma was detected by Transwell assay;the expression of Nav1.5 m RNA in oral squamous cell carcinoma was detected by RT-PCR;Western blot was used to detect oral squamous cell carcinoma Protein expression in Nav1.5.Results The results showed that different concentrations of TTX acted on oral squamous cell carcinoma,and the proliferation of SCC3 cells in oral squamous cell carcinoma did not show significant differences between the groups,and the difference was not statistically significant.No significant changes in cell morphology were observed during the TTX action.After 24 h of TTX with 1umol/L and 30umol/L,the invasive rate of SCC3 cells was decreased by(11.50±4.5)% and(47.38±4.6)%, respectively,compared with the untreated TTX group,and the difference was significant(P< 0.05).The relative expression level of Nav1.5 protein in oral squamous cell carcinoma cells was(0.63±0.02),and after 24 hours of adding 30 μmol/L TTX,the relative protein expression was(0.32±0.01),the difference was statistically significant(P<0.05).In RT-PCR,the cellular m RNA was reduced by 56% after treatment with 30umol/L TTX for 24 h,which was significantly different from that of untreated cells(P<0.05).Conclusions Nav1.5 is highly expressed in oral squamous cell carcinoma SCC3 cells and inhibits the invasive ability of oral squamous cell carcinoma cells by blocking voltage-gated sodium channels through TTX to reduce Nav1.5 expression.