Analysis Of Smoke Biomarkers And Oxidative Stress Markers In Urine Of Different Smoker

The chemical substances released by tobacco burning have a greater impact on health.Among them,tobaccos represented by NNK,NNN,NAB,and NAT are unique to nitrosamines,polycyclic aromatic hydrocarbons represented by benzo[a]pyrene,and tobacco organisms represented by nicotine.Alkali is closely related to health.As the number of smokers gradually increases,the exposure to harmful components of smoke also increases,causing great health risks[1,2].Cigarette smoke is the main source of free radicals during smoking.It promotes the production of oxidative stress by directly delivering reactive oxygen species and by activating inflammatory cells.8-Hydroxydeoxyguanosine(8-OHdG)and 8-hydroxyguanosine(8-OHG)are oxidative damage biomarkers of DNA and RNA,respectively[3,4].To understand in depth the effects of smoking on health,it is necessary to fully understand the metabolism of harmful components of smoke in the body.In view of the extremely low content of these components in smokers,the complex matrix,poor thermal stability,and complex pretreatment and other technical difficulties,this experiment took different smokers’ urine as the research object and adopted different methods to achieve the uniqueness of tobacco in urine.Accurate quantitative analysis of nitramine,benzo[a]pyrene,nicotine and their oxidative stress markers.Objective:Accurate quantitative analysis of smoke exposure biomarkers(TSNAs,BaP and nicotine)and oxidative stress markers(8-OHdG and 8-OHG)in urine of different smoking populationsMethod:Chapters 1 and 2 use self-packed solid-phase extraction columns and tail-tube sampling bottles to enrich and concentrate the urine of different smoking populations.The collected extracts are directly filtered by LC-MS/MS and HPLC.-FLD analysis of TSNAs,BaP and metabolites in urine.The third chapter independently designed the sample extraction and filtration device to realize the integration of the filtration and sampling of nicotine and cotinine in urine samples,and the use of LC-MS/MS direct sampling analysis.Chapter 4 for the determination of urine 8-OHdG and 8-OHG in different smokers,respectively optimized the sample preparation conditions,chromatographic conditions and mass spectrometry conditions,to achieve accurate quantitative analysis of oxidative stress markers in the urine of smokers.Results:In the context of smoking and health,the characteristics of TSNAs,benzopyrene,nicotine and their metabolites,as well as the biomarkers of oxidative stress 8-hydroxydeoxyguanosine and 8-hydroxyguanosine,are The urine of different smoking populations is the research object.The pretreatment experiment optimization,chromatographic conditions and mass spectrometry conditions of urine samples were optimized to achieve accurate quantitative analysis,which provided a reliable experimental basis for evaluating the impact of smoking on human health.Conclusion:The levels of TSNAs,nicotine and its metabolites and oxidative stress markers 8-OHdG and 8-OHG in the urine of different smokers have a certain correlation with the degree of smoking,the order is:heavy smokers>moderate-smokers>mild-Smokers>Non-smokers.It shows that the determination of tobacco-specific nitrosamines,nicotine and its metabolites,as well as the oxidative stress markers 8-OHdG and 8-OHG can be used as a basis for the preliminary judgment of human cigarette smoke exposure levels;However,the differences in the contents of B[a]P,1-OHP,3-OHB[a]P in urine samples of different smoking populations are not obvious,and there is no obvious rule,indicating that the determination of B[a]P and its metabolites cannot be used as a judgment basis for exposure levels of cigarette smoke in humans.