Alteration Of Gut Microbiota And Metabolites In Coronary Artery Disease And Effects Of Different Microbiota Structure On The Development Of Colorectal Cancer

Alteration of gut microbiota and metabolites in coronary artery diseaseBackground:Coronary artery disease(CAD)is a common cardiovascular disease Many studies have found changes in intestinal microbiota and metabolites in the patients,but few studies can explain their correlationMethods:We included 37 patients with coronary heart disease and 37 healthy controls,and collected their fresh feces and venous blood.16S rDNA sequencing was applied to detect the microbiota and non-targeted metabolomics was used to detect serum metabolites.Bioinformatics analysis and correlation analysis were used to find the alteration in microbiota and metabolites and their associations,and build diagnostic models.Results:Compared with healthy control group,the richness of gut microbiota in CAD decreased significantly and the composition of the microbiota changed.The abundance of Bacteroides,Flavonifractor and Oscillibacter increased in CAD,while the abundance of Blautia,Prevotella,Streptococcus decreased.Lipid-related metabolites and exogenous metabolites were the major changes in the CAD patients.Fatty acid metabolism was significantly reduced while some drug-related metabolites were elevated.The result revealed that certain bacteria might affect atherosclerosis by modulating the metabolic pathways of the host,such as fatty acid and benzene metabolism.Using differential microbiota and metabolites,the diagnostic model also showed probability in identifying those who suffered from CAD.Conclusion:Our study successfully identified the dysbiosis signature of gut microbiota and circulation metabolites and their potential relationship in the patients with coronary artery disease.Our study provided additional evidence for understanding the interaction of host-gut microbiota in the pathogenesis of atherosclerosisEffects of different microbiota structure on the development of colorectal cancerObjective:To investigate the differences in gut microbiota of people at different ages,and to study the effect of different structure of the gut microbiota on the occurrence and development of colorectal cancer.Methods:We collected stool samples from a total of 112 people from 4 different age groups.,analyzed the structure and composition of the microbiota,and gavage these fecal microbiota to the colorectal cancer model mice to observe the formation of intestinal tumors in mice.The intestinal flora of different groups of mice was compared to find specific bacteria related to the development of colorectal cancer.Results:The richness of microbiota in the four age groups increased,and there were significant differences in the diversity of the microbiota among the groups.The abundance of the beneficial bacteria Bifidobacterium,Roseburia,Faecalibacterium decreased with age,and the abundance of Alistipes,Parabacteroides,Prevotella increased.After the mice of each group were administrated with fecal fluid,the intestinal tumors of the mice showed a gradual deterioration trend.Further analysis of the gut microbiota composition in mice revealed that the structure of gut microbiota of the mice in each group also had significant differences.The changes in the abundance of Faecalibacterium may be related to the occurrence and development of colorectal cancer.Conclusion:There are significant differences in the composition of gut microbiota in people at different ages,and the reduction of beneficial bacteria and the increase in the abundance of possible pathogenic bacteria can affect the occurrence and development of colorectal cancer.