Analysis The Intestinal Flora Of Spontaneous Tumor In Tree Shrew Based On 16SrRNA Sequencing

Objective:Tumor research has become an important part of biomedicine development Animal models are widely used in tumor development,treatment and anti-tumor drug research.There are about 22 063 protein coding genes in the genome of tree shrew,and the number of genes is closer to human than other experimental animals.Compared with rodents,tree shrews are more closely related to primates.Primates are very closely related to humans and can better simulate the occurrence and development of human diseases.It has been found that induced intraductal papilloma and invasive ductal carcinoma of tree shrew are common pathological types of human tumors,and spontaneous tumors can better simulate the characteristics of human tumors.At present,there are few studies on the relationship between intestinal microflora and tumor diseases in tree shrews.In this paper,high-throughput sequencing was used to analyze the intestinal flora of two kinds of spontaneous tumors in tree shrew,to understand the changes of intestinal microflora structure and diversity.To explore the changes of intestinal flora of spontaneous high-grade sarcoma and spontaneous intraductal papillary neoplasms in tree shrews.This study provides basic data for the correlation between tumorigenesis and intestinal floraMethods:This experiment was divided into groups according to the results of HE pathological staining of tree shrews tumor tissues.In this study,three tree shrews with spontaneous high-grade sarcoma were selected as CA1 group,four tree shrews with intraductal papillary neoplasms were selected as CA2 group,and 6 healthy tree shrews were selected as healthy control group(NO).Through shear filtering of Reads,OTUs clustering,database comparison,annotation analysis and abundance analysis of intestinal flora,the intestinal flora structure,abundance and diversity of each group of tree shrews were obtainedResults:There were 494 OTUs in CA1 group,449 species in Ca2 group and 640 species in no group.OTUs diversity of healthy tree shrew was higher than CA1 and Ca2 group.The abundance of Proteobacteria,Firmicutes,Bacteroidetes and actinobacteria were higher than CA1 and Ca2 group.Compared with CA1 group and Ca2 group,the No group diversity index Chaol index value,ACE index value,Shannon index value had no statistical significance(P>0.05).The Biomarker of CA1 group was Lactobacillus.The main biological markers in Ca2 group were Flavobacterium,gamma Proteobacteria,Pseudomonas dales and moraxellaceaeConclusion:The spontaneous tumors of tree shrews mainly occurred at the age of 5-7 years.The abundance and diversity of intestinal microflora in high-grade sarcoma and intraductal papillary neoplasms are slightly lower than control group,and the biological markers of the two tumors are different.These results suggest that the changes of intestinal flora structure and abundance are closely related to the occurrence and development of high-grade sarcoma and intraductal papillary neoplasms.