| Objective FAM134B gene product is a kind of endoplasmic reticulum autophagy protein.At present,the research shows its function in many aspects.Its function and expression have tissue organ specificity and disease specificity.It plays a certain role in sensory nerve disease,vascular disease,viral disease and tumor.It has different characteristics in different tumors,for example,it shows the role of oncogenes in esophageal cancer,but in colorectal cancer and breast cancer showed the role of tumor suppressor genes.The role of FAM134B in HCC is rarely studied.In this paper,we will expand the expression of FAM134B in HCC,its relationship with clinicopathological features and the influence on prognosis.We will study the influence of the overexpression model of FAM134B in vitro on the proliferation,migration,invasion,apoptosis and other biological functions of HCC cell lines.And explore the main functions of its related genes in liver,so as to speculate its role in HCC.It provides a new idea for the early diagnosis and treatment of HCC.Methods Firstly,the expression of FAM134B in tumor and normal livertissues was detected by immunohistochemistry and Western blotting.The transcription and clinical information data of HCC in TCGA database were analyzed.The expression of FAM134B in gene level was analyzed.The correlation between FAM134B level and clinicopathological parameters and the influence on prognosis of HCC were analyzed.The overexpression model of fam134b in hepatoma cells was established.The effects of FAM134B overexpression on cell proliferation,migration,invasion and apoptosis were observed.and finally to analyze the information of FAM134B related genes by GO and KEGG enrichment analysis.Results The results of immunohistochemistry showed that FAM134B was mainly located in the cytoplasm of liver tissue.The results of WB showed that the expression of FAM134B in HCC was lower than that in normal livertissues.TCGA database analysis showed that fam134b gene level was lower in HCC.The patients in the low level group of FAM134B were younger,more Asian,worse differentiated,more cirrhosis and higher AFP.At the same time,the prognosis of low-level patients is worse.FAM134B level,T stage,age,pathological grade and hepatitis B index were independent risk factors for prognosis of HCC.The overexpression of FAM134B can inhibit the proliferation,migration,invasion and promote the apoptosis of HepG2 cells.FAM134B related genes in liver were enriched in 4 GO terms,poly(A)RNA binding,intracellular ribonucleoprotein complex,organelle membrane and telomerase holoenzyme complex.These related genes were mainly enriched in metabolic pathways,biosynthesis of antibiotics,carbon metabolism,ribosome and drug metabolism terms.These terms are more related to protein metabolism.Conclusion FAM134B is low expression in HCC,which is related to many malignant phenotypes.The prognosis of patients with low expression is worse,and FAM134B level is an independent risk factor for prognosis of patients with HCC.Overexpression of FAM134B can inhibit the biological activity of HepG2 and promote their apoptosis in vitro.FAM134B related genes are mostly enriched in the terms related to protein metabolism.In conclusion,FAM134B is expected to be a new early diagnosis and treatment target of HCC. |
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